Protein Info for SO1820 in Shewanella oneidensis MR-1

Name: polB
Annotation: DNA polymerase II (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 PF03104: DNA_pol_B_exo1" amino acids 174 to 308 (135 residues), 36.1 bits, see alignment E=7.1e-13 PF00136: DNA_pol_B" amino acids 400 to 781 (382 residues), 156.3 bits, see alignment E=1.8e-49

Best Hits

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 100% identity to son:SO_1820)

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EFZ4 at UniProt or InterPro

Protein Sequence (808 amino acids)

>SO1820 DNA polymerase II (NCBI ptt file) (Shewanella oneidensis MR-1)
MLTRHAIARDGNLLLQYYLATESGPVLMELPDTEYICFCHQTDIQALQLQTPGLALRFVP
LELKSFKRQAVAGIYAKSSSAFRSLQRIANDAGIPLFEADIRPEQRFLIERFVALDVAFL
GHYANKIANNIKCDNDSAIQAINKSENSQGHLSVFTAMRAKAIAPQPTIKLRSISLDFEC
SFDGLLYSVALYGRDAQAQPYEKVIMVGEVQSDAPQYIEWVSDEAMLIRRLIAWFAEFDP
DVIIGWSVVTFDLALLYRRALLHRIPLRLGRGGALLEWKVDNKFRPETLNLPGRVVLDGI
DWLKAAFYHFERFSLEFVAQALLGEGKAIHDVENRVQEIDALFTEDKQSLAHYNLTDSRL
VWDIFEHTQLWDFALARAELTGLELGRVGASVAAFNHLYLPHLHRAGYVAPAEPASQGIE
SPGGYVMDSVPGFYQHILVFDFKSLYPSIIRTFLIDPKGLIEGLGDDSLVLDESETVTGF
LGARFSRRQPILPKLIQTLSEQREKAKREANAPLSQAIKIIMNSLYGVLGSQGCVFHDAK
LASSITMRGHQIMKQTRAWIEEMGYQVIYGDTDSTFVHLGYEPDLSDISALGKQIAAQIN
ANWQAKITKDFELESFLELQFERHYEQFFMPTLRGSEEGSKKRYVGAWRNVSGELEITFK
GMEQVRSDWSPLARKVQAELYERMFNQRDISGYLADVIGELQAGKRDDELIFSKRMRRNL
EEYTAKSSPHVKVARQLCELTGKASYSKRGAQIDYVITVNGPEPVSHRSSTIDYQYYIDK
QIGPIAEPVFSIMKLNYTNIASKQLSLI