Protein Info for SO1521 in Shewanella oneidensis MR-1

Annotation: iron-sulfur cluster-binding protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 914 PF01565: FAD_binding_4" amino acids 22 to 159 (138 residues), 98.1 bits, see alignment E=1.8e-31 PF02913: FAD-oxidase_C" amino acids 249 to 496 (248 residues), 178.1 bits, see alignment E=1.3e-55 PF13183: Fer4_8" amino acids 519 to 591 (73 residues), 40 bits, see alignment E=2.3e-13 PF00037: Fer4" amino acids 520 to 537 (18 residues), 22.8 bits, see alignment (E = 3.1e-08) PF12800: Fer4_4" amino acids 520 to 534 (15 residues), 15.7 bits, see alignment (E = 8e-06) amino acids 576 to 590 (15 residues), 13.4 bits, see alignment (E = 4.3e-05) PF13534: Fer4_17" amino acids 521 to 591 (71 residues), 32.2 bits, see alignment 6.2e-11 PF12838: Fer4_7" amino acids 521 to 591 (71 residues), 34.9 bits, see alignment 8.4e-12 PF02754: CCG" amino acids 687 to 763 (77 residues), 13.8 bits, see alignment E=2.7e-05 amino acids 814 to 880 (67 residues), 18.3 bits, see alignment 1e-06

Best Hits

KEGG orthology group: None (inferred from 100% identity to son:SO_1521)

MetaCyc: 100% identical to D-lactate dehydrogenase Dld-II (Shewanella oneidensis)
D-lactate dehydrogenase (cytochrome c-553). [EC: 1.1.2.5]

Predicted SEED Role

"Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing" in subsystem Lactate utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EGS3 at UniProt or InterPro

Protein Sequence (914 amino acids)

>SO1521 iron-sulfur cluster-binding protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MTDDPVRRFAWSTDASYFRIVPEVVVHAETLEQVKLTLTVARKHNAPVTFRAAGTSLSGQ
AIGEGILLILGHDGFRKIEVSSDAKQITLGAAVIGSDANAVLAPLNRKIGPDPATIASAK
IGGIVANNASGMCCGTAQNSYQTIASAKLLFADGTELDTGCEKSKAEFAKTHGKLLQDLS
ELSHLTRHNSALAERIRKKYSIKNTTGYGINSLIDFTDPFDIINHLMVGMEGTLAFINEV
TYHTVNEAKFKASAMAVFHNMEDAARAIPLINGESVSAAELLDWPSIKAVTGKPGMPDWL
SELPALSAILLIESRADDAQTLEHYTQDVTAKLAGFDFIRPMEFSTNPAVYDKYWAMRKG
LFPIVGGERPKGTSVIIEDVAFELEHLAAAAHDITELFHKHGYPEGCIYGHALAGNFHFI
ITPAFTTQADIDRFHAFMDDIADMVINKYNGSMKAEHGTGRAVAPFVEKEWGQDAYTLMK
NIKQVFDPQGILNPGVILNDDSNIHVKNIKPCPVVDDFVDKCIECGFCEKTCPTSALNFS
PRQRIATLREIERLEQSGDKAAAAKMRADAKYDVIDTCAACQLCTIACPVDNSMGQLVRK
LRTPYISTTEQKVLDFQAKHFGAVNQVISTGFDVLGVIHKITGDGITNALMKTGRLISKE
VPYWNPDFPKGGKLPKPSPAKAGQETVVYFPACGGRTFGPTPKDPDNRTLPEVVVTLLER
AGYNVITPEKTRDLCCGQMWESKGDFKNADAKRQELIDVLSKMSNGGKIPVLVDALSCTY
RTLTGNPQVQITDLVEFMHDKLLDKLSINKKVNVALHLGCSARKMKLEPKMQAIANACSA
QVLKPAGIECCGYAGEKGLYKPEINASALRNIKKLIPVEVKEGYYANRMCEVGLTQHSGI
SYRHLAYLLEECSR