Protein Info for SO1368 in Shewanella oneidensis MR-1
Name: pepA-2
Annotation: cytosol aminopeptidase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AMPA2_SHEON: Probable cytosol aminopeptidase 2 (pepA2) from Shewanella oneidensis (strain MR-1)
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to son:SO_1368)MetaCyc: 76% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- glutathione degradation (DUG pathway) (2/2 steps found)
- γ-glutamyl cycle (4/6 steps found)
- muropeptide degradation (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.1, 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8EH62 at UniProt or InterPro
Protein Sequence (502 amino acids)
>SO1368 cytosol aminopeptidase (NCBI ptt file) (Shewanella oneidensis MR-1) MEFSVKSGSPEKQRSACIVVGVYEPRRLSGIAEQLDKISEGYISNLLRRGDLEGKPGQML LLHHVPNVLSERVLLVGCGKERELDERQYKQIITKTINTLNETGSMEAVCFLTELHVKGR DTYWKVRQAVETTNSSLYCFDALKTRKGETRRPLRKLVFNVPTRRELTLGERAIEHGMAV SSGMHLCRDVANMPPNICNPAYLASQARQLAEIHENLHVSTVGEEQMAKLGMNSYLAVGR ASANESIMTVMEYKGAVDSTEKPIVLIGKGLTFDSGGISLKPGEAMDEMKYDMGGAAGVI GTMKAICEMKLPINVVGILAGCENMPSGNAYRPGDILTTLSGQTVEVLNTDAEGRLVLCD VLTYVERFDPELVIDTATLTGACVIALGKHASGLFSSHNPLAHELLNAGEQSGDRAWRMP LWDEYQDMLDSPFADMTNLGGRPAGAITAACFLSRFAKKYNWAHLDVAGTAWNSGANKGS TGRPVPILTQFLINRAGVELGE