Protein Info for SO1349 in Shewanella oneidensis MR-1

Name: era
Annotation: GTP-binding protein Era (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 44 to 206 (163 residues), 106.7 bits, E=1.1e-34
• TIGR00436: GTP-binding protein Era
  • amino acids 46 to 319 (274 residues), 341.4 bits, E=3.6e-106
• PF02421: FeoB_N
  • amino acids 48 to 205 (158 residues), 42.4 bits, E=1.8e-14
• PF01926: MMR_HSR1
  • amino acids 48 to 164 (117 residues), 93.5 bits, E=3.2e-30
• PF00009: GTP_EFTU
  • amino acids 48 to 213 (166 residues), 30 bits, E=1.4e-10
• PF04548: AIG1
  • amino acids 49 to 179 (131 residues), 29.9 bits, E=1.2e-10
• PF07650: KH_2
  • amino acids 248 to 324 (77 residues), 67.5 bits, E=2.6e-22

Best Hits

Swiss-Prot: 69% identical to ERA_HAES1: GTPase Era (era) from Haemophilus somnus (strain 129Pt)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 99% identity to shn:Shewana3_3025)

MetaCyc: 66% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q8EH80 at UniProt or InterPro

Protein Sequence (339 amino acids)

>SO1349 GTP-binding protein Era (NCBI ptt file) (Shewanella oneidensis MR-1)
MTKKTDLPAVDAANASNEPSLDELLARMNAASAAAPSVHYDVTYCGMVAIIGRPNVGKST
LLNRLLGQKISITSKKPQTTRHRIMGIHTDGPRQIVFIDTPGLHIEEQRAINRLMNRAAA
SSLADVSMVIFVVDGMTWTADDEMVLSKLRRGGEERKTVLAINKVDNIKDKEALFPYLED
VAKKYPFDEILPISASKGTNVKRILELAAQSLPENPFFFPEDYVTDRSQRFMASEIVREK
LMRFLGDELPYDATVEIEQFKMMENGVYQINALILVEREGQKRMVIGSKGERIRTIATQA
RLDMETLFDNKVFLEVWVKVKSGWADDERALRSLGYGDD