Protein Info for SO1268 in Shewanella oneidensis MR-1

Updated annotation (from data): Glutamate--putrescine ligase (EC 6.3.1.11)
Rationale: Specifically important for utilizing Putrescine Dihydrochloride. Automated validation from mutant phenotype: the predicted function (RXN0-3901) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: glutamine synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 111 to 445 (335 residues), 360.8 bits, E=3.7e-112

Best Hits

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to son:SO_1268)

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)
• L-aspartate degradation II (aerobic) (1/3 steps found)
• L-aspartate degradation III (anaerobic) (1/3 steps found)
• superpathway of L-arginine and L-ornithine degradation (8/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (6/11 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See Q8EHF4 at UniProt or InterPro

Protein Sequence (451 amino acids)

>SO1268 Glutamate--putrescine ligase (EC 6.3.1.11) (Shewanella oneidensis MR-1)
MNKLIAFLKERKITEVECVICDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVD
DDIYYSLLDDADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKV
LSLYDEKGWEPVIAPEMEFYLTSRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPL
FEDMYDWCEIQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFVFKRTLREAALKHNVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTQSALFLSYIAGLQKYIPEFLPL
MAPNANSFRRFLPGTSAPVNLEWGIENRTCGLRIPESSPQNRRIENRIPGADANCYLAFA
AGLLCGYIGMVEGLKPSTPVQGKANESRSNNPHCLPLTLEEALVAMEESDACKEYLGESF
TTGFVAVKQAELENFRRVVSSWEREFLLLSV