Protein Info for SO1258 in Shewanella oneidensis MR-1
Annotation: adenylosuccinate synthetase, putative (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to PURA2_PSEHT: Adenylosuccinate synthetase 2 (purA2) from Pseudoalteromonas haloplanktis (strain TAC 125)
KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 100% identity to son:SO_1258)Predicted SEED Role
"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotide salvage (13/14 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.3.4.4
Use Curated BLAST to search for 6.3.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8EHG3 at UniProt or InterPro
Protein Sequence (418 amino acids)
>SO1258 adenylosuccinate synthetase, putative (NCBI ptt file) (Shewanella oneidensis MR-1) MPSIVVVGANWGDEGKGRIVDYLAADAAASVRFQGGNNAGHTVVNDYGTFKLHQLPSGIF NPNCVAVLGPGMVISPEKLSVEIAEVEAAGIEVKLCISDRATLCLPIHALEDTLEEQRLG DLAYGSTRQGIAPAYGDRVMKKGILVGWLKQPEVLQERIQFMLNWKLPQLKALYPSCDFS QTAAEMTQWLLDVTAPWRPFICNVTEPLKALQKQDANLLFEAQLGAGRDLIYGEYPYTTS SNVTAAFAGLGSGLPALRPERVIAVAKAFSSSVGTGTLVTAMEEQDDFRKNANEFGATTG RPRDMGYFDAVATRNGVELQAATEIALTKIDCLTGMKDLKICIAYEGEHTENPIWPQTAA LSPVYEQMESWSEDITTCRTFESLPKAAQHYVERIEALMGVRVSMVSVGPERDQMIIR