Protein Info for SO1142 in Shewanella oneidensis MR-1

Name: carB
Annotation: carbamoyl-phosphate synthase, large subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1052 (1051 residues), 1578.5 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 245.6 bits, E=1.6e-76
  • amino acids 673 to 874 (202 residues), 130.1 bits, E=3.4e-41
• PF02787: CPSase_L_D3
  • amino acids 427 to 504 (78 residues), 92.7 bits, 4.5e-30
• PF02655: ATP-grasp_3
  • amino acids 672 to 845 (174 residues), 34.1 bits, E=1.2e-11
• PF02222: ATP-grasp
  • amino acids 680 to 845 (166 residues), 33.3 bits, E=1.6e-11
• PF02142: MGS
  • amino acids 956 to 1041 (86 residues), 72.5 bits, E=1e-23

Best Hits

Swiss-Prot: 70% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 74% identity to abo:ABO_0318)

MetaCyc: 83% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q8EHS5 at UniProt or InterPro

Protein Sequence (1074 amino acids)

>SO1142 carbamoyl-phosphate synthase, large subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM
ADATYIEPIQWEVVRNIIAKERPDAILPTMGGQTALNCALELEAKGVLAEFNVEMIGATA
DAIDKAEDRSRFDKAMKSIGLECPRAGIAHSMEEAYGVLEQVGFPCIIRPSFTMGGSGGG
IAYNKEEFEEICSQGLDLSPTKELLIDESLIGWKEYEMEVVRDRNDNCIIVCSIENFDPM
GVHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFGINPKDGRMVIIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGFTLDELMNDITGGRTPASFEPAIDYVVTKVP
RFNFEKFAGSNDRLTTQMKSVGEVMAIGRTFQESLQKALRGLEVNRHGLDPITDMSKPEA
MARIRLELKEPGCDRIWYIADAMRAGLTLDEIFRLTNIDPWFLVQIEELIKLEGQVAEAG
LAGLNEELLRKLKRKGFADARLAVVLGVNESEVRKLRDRFDIHPVYKRVDTCAAEFATDT
AYMYSTYEEECEANPSNRDKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPI
IGTSPDAIDRAEDRERFQQAIQRLEMKQPENDTVTTVEGAVIAAERIGYPLVVRPSYVLG
GRAMEIVYDQQDLLRYFNEAVSVSNASPVLLDHFLDDAIEVDIDAVCDGETVVIGAIMEH
IEQAGVHSGDSGCSLPPYTLSQEIQDRMRVQVGKLAMELGVVGLMNVQFAVKNNEIYMIE
VNPRAARTVPFVSKATGVPLAKIAARVMAGQSLKAQNFTQEVIPPFYSVKEVVLPFNKFP
GVDPLLGPEMRSTGEVMGVGDTFAEAYAKAQLGATSEVPKSGRALLSVRNSDKKRVADLA
AKLIELGYQIDATHGTAVILGEAGINPRLVNKVHEGRPHILDRIKNGEYTYIVNTTEGRQ
AIEDSRQLRRGALRYKVNYTTTMNAAFATCMAHAADDRTNVTSVQELHKRILAK