Protein Info for SO1126 in Shewanella oneidensis MR-1

Name: dnaK
Annotation: chaperone protein DnaK (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR02350: chaperone protein DnaK" amino acids 3 to 601 (599 residues), 944.2 bits, see alignment E=1.3e-288 PF00012: HSP70" amino acids 4 to 600 (597 residues), 881 bits, see alignment E=3.5e-269 PF06723: MreB_Mbl" amino acids 115 to 376 (262 residues), 45.3 bits, see alignment E=5.7e-16

Best Hits

Swiss-Prot: 100% identical to DNAK_SHEON: Chaperone protein DnaK (dnaK) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to son:SO_1126)

MetaCyc: 80% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EHT7 at UniProt or InterPro

Protein Sequence (639 amino acids)

>SO1126 chaperone protein DnaK (NCBI ptt file) (Shewanella oneidensis MR-1)
MGKIIGIDLGTTNSCVAVLDGGKARVLENAEGDRTTPSIIAYTDDETIVGQPAKRQAVTN
PNNTFFAIKRLIGRRFKDDEVQRDVNIMPFKIIAADNGDAWVESRGNKMAPPQVSAEILK
KMKKTAEDFLGEEVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAALAYGI
DKKQGDNIVAVYDLGGGTFDISIIEIDSNDGDQTFEVLATNGDTHLGGEDFDNRLINYLA
DEFKKEQGLDLRKDPLAMQRLKEAAEKAKIELSSTNQTEVNLPYITADATGPKHLVVKIT
RAKLESLVEDLIIRTLEPLKVALADADLSVSDINEVILVGGQTRMPKVQEAVTNFFGKEP
RKDVNPDEAVAVGAAIQAGVLSGDVKDVLLLDVTPLSLGIETMGSVMTKLIEKNTTIPTK
AQQVFSTADDNQSAVTIHVLQGERKQASANKSLGQFNLDGIEPAPRGMPQIEVMFDIDAD
GILHVSATDKKTGKKQNITIKASSGLSEEEVAQMVRDAEAHAEEDKKFEELVQSRNQADG
LVHATKKQVEEAGDALPSEDKAKIEAAMSAVETAVKGNDKEAIEKATQALIEASAKLMEI
AQAKAQTQGGAQEGAAKQSNATADDVVDAEFEEVKDDKK