Protein Info for SO1030 in Shewanella oneidensis MR-1

Name: metH
Annotation: 5-methyltetrahydrofolate--homocysteine methyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 20 to 1209 (1190 residues), 1757.7 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 24 to 336 (313 residues), 329.4 bits, E=7e-102
• PF00809: Pterin_bind
  • amino acids 371 to 609 (239 residues), 211 bits, E=5.7e-66
• PF02607: B12-binding_2
  • amino acids 675 to 747 (73 residues), 89 bits, 4.5e-29
• PF02310: B12-binding
  • amino acids 762 to 851 (90 residues), 64.2 bits, 2.5e-21
• PF02965: Met_synt_B12
  • amino acids 954 to 1225 (272 residues), 427.8 bits, E=4.6e-132

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 63% identity to azc:AZC_4341)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• folate transformations II (plants) (11/11 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• folate transformations I (10/13 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• superpathway of L-methionine salvage and degradation (4/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See Q8EI23 at UniProt or InterPro

Protein Sequence (1244 amino acids)

>SO1030 5-methyltetrahydrofolate--homocysteine methyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MAISPNRASHTLADIRNQLSTRILILDGAMGTMIQGYKLEEADYRGERFKDWHTDVKGNN
DLLVLTQPHIIKQIHTDYLLAGADIIETNTFNATTIAMADYDMQSLSAEINREGARLARE
ACDAIEQATGKPRYVAGVLGPTNRTCSISPDVNDPGFRNIHFDELVTAYCESTRALIEGG
ADIIMVETIFDTLNAKAALFAIETVFDELFGANSPARLPVMISGTITDASGRTLTGQTTE
AFYNSLRHIKPLSIGLNCALGPKELRPYVEELSRIAECYVSAHPNAGLPNEFGGYDETPE
DMAKVIQEWAREGMLNIIGGCCGSTPEHIKVIREAVEQFAPRVLPEIPVACRLAGLEPLT
IDAQTLFVNVGERTNVTGSAKFLKLIKEGKFEQALDVAREQVESGAQIIDINMDEGMLDG
VEIMHKFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISLKEGEEKFIEQ
ATLVKRYGAAAIIMAFDEQGQADTKARKVEICTRAYRVLVDKVGFPPEDIIFDPNIFAIA
TGIDEHDNYAVDFIDAIKEIKATLPHAMISGGVSNVSFSFRGNNPVREAIHAVFLYHAIK
VGMDMGIVNAGQLAIFDDIDPELKVRVENVVLNLPCPVEGSNNTEQLLEIAEKFRGDGSS
SAKKEDLEWRSWPVNQRLAHALVKGITEFIDEDTEAARQAASRPLDVIEGPLMDGMNIVG
DLFGSGKMFLPQVVKSARVMKKAVAYLNPFIEKEKVAGQSNGKILMVTVKSDVHDIGKNI
VGVVLACNGFEVFDLGVMVSVERILEAVKEHNIDIIGMSGLITPSLDEMVHNVKTFHREG
LTIPAIIGGATCSKIHTAVKIAPHYPHGAIYIADASRAVPMVSKLVNNETRQATIDETYA
EYDDMRTKRLSQAKRKEIVSLEAARENRCQHDWANYSPFKPNVLGRQVFDDYPLTDLVDR
IDWTPFFRAWELHGHYPEILSDKVVGVEAQKLFSDGKAMLKKIIEEKWLTAKGVIGLFPA
NTVGFDDIELYTDETRTEVELTTHHLRMQLERVGNDNFCLADFVAPKDSGVADYMGGFAV
TAGHGIDEHVARFEANHDDYNAIMLKCLADRLAEAFAERMHERVRKEFWGYAADEQLDNE
ALIREKYKGIRPAPGYPACPDHTEKGLLWELLKPNETIDLNITESYAMFPTAAVSGWYFA
HPKSRYFGVSNIGRDQVEDYAKRKGMTVAETEKWLAPVLDYDPE