Protein Info for SO1016 in Shewanella oneidensis MR-1

Name: nuoG
Annotation: NADH dehydrogenase I, G subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 909 PF13510: Fer2_4" amino acids 3 to 78 (76 residues), 43 bits, see alignment E=9.7e-15 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 657 (654 residues), 669 bits, see alignment E=4.1e-205 PF00111: Fer2" amino acids 5 to 70 (66 residues), 30.1 bits, see alignment 9.6e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 127 (40 residues), 49.4 bits, see alignment (E = 6.6e-17) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 49.5 bits, see alignment 8.6e-17 PF00384: Molybdopterin" amino acids 281 to 640 (360 residues), 75.7 bits, see alignment E=9.4e-25

Best Hits

Swiss-Prot: 100% identical to NUOG_SHEON: NADH-quinone oxidoreductase subunit G (nuoG) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 89% identity to avr:B565_2350)

MetaCyc: 64% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EI34 at UniProt or InterPro

Protein Sequence (909 amino acids)

>SO1016 NADH dehydrogenase I, G subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MATIYVDGKEYEVDGADNLLQACLSLGLDVPYFCWHPALGSVGACRQCAVKQYQNADDKR
GRLVMSCMTPSTDGTYISIEDEEAKEFRKCVVEWQMTNHPHDCPVCEEGGACHLQDMTVM
TGHNSRRYRFTKRTHQNQDLGPFINHEMNRCIACYRCVRYYKDYAGGEDLGVYGAHDNVY
FGRVEDGTLESEFSGNLVEVCPTGVFTDKTHSERYNRKWDMQFAPSICQQCSVGCNISPG
ERYGELRRIENRFHGSVNHYFLCDRGRFGYGYVNLPDRPRQPQLKNGNEQLAITVDGALN
RAADALRNAKGVIGIGSPRASLESNFALRELVGANNFYVGVEQAEWQCQQQMLHILQHGG
VPTPSLRDMEEADAILVLGEDVTMSAARIALSLRQAVKGKARELARKMKVDLWQVAAVQT
LGQNQRYPLLITSLDTTRLDDVVEDKLHAPYADQARLGFAIANLLDPAAPAVTDLGAEQQ
AQAARWAELLGNAKKPLIIAGSSARSMALIEAASNIARALKGRGLEASIALVGQEANSLG
LAMLASTSQPLEAALERIEAEEGLALVTVENDLYRRAARNRVDAALARLQHLLVIDHQAT
STAQKADLVLPAASFAETDGTLVNMEGRAQRFFQVYAPAFYNADIQVREGWRWLAALQGT
LEHKPLRWQTFDEISHACAASVPLLATMLEAAPNAGLRIRGMRLAREPHRYSGRTSMLAN
QNVSEPRVAQDPDSPFNFSMEGYAGSRQDMPQVPFAWAPGWNSPSAWNKFQDEVGGHLRA
GDPGRRLLEATADTLDWFSAIPAPFKTADALQVVNYAQLFGGEELSARSPVIQARMSEPM
LVLNPHDAARFALVEGGLVAFSWGGNHWRLPLRLSKKLSQGLVGLPLGVSGLPTALHQAY
LANLQEAIA