Protein Info for SO0872 in Shewanella oneidensis MR-1

Name: pcnB
Annotation: polyA polymerase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 TIGR01942: poly(A) polymerase" amino acids 4 to 435 (432 residues), 682.5 bits, see alignment E=1.1e-209 PF01743: PolyA_pol" amino acids 34 to 163 (130 residues), 122.8 bits, see alignment E=1.8e-39 PF12627: PolyA_pol_RNAbd" amino acids 191 to 251 (61 residues), 71.9 bits, see alignment E=4.3e-24 PF12626: PolyA_pol_arg_C" amino acids 308 to 426 (119 residues), 136.9 bits, see alignment E=5.3e-44

Best Hits

Swiss-Prot: 59% identical to PCNB_ECOL6: Poly(A) polymerase I (pcnB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 100% identity to son:SO_0872)

MetaCyc: 59% identical to poly(A) polymerase I (Escherichia coli K-12 substr. MG1655)
Polynucleotide adenylyltransferase. [EC: 2.7.7.19]

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EIG7 at UniProt or InterPro

Protein Sequence (438 amino acids)

>SO0872 polyA polymerase (NCBI ptt file) (Shewanella oneidensis MR-1)
MALVPRDAHSISRKQISENALKVLYRLNKAGFQAYLVGGGVRDLLLGLEPKDFDVVTNAT
PEEIKKLFRNCRVVGRRFRLAHIVFGRDVIEVATFRGHHGESNEKISKANAEGRLLRDNV
YGEIDEDAERRDFTVNALYYDISDYSIRSYGGGMEDLKAGTLRLIGDPETRYREDPVRML
RAVRFATKLSMTIDEVTAKPIKQLAPLLKDIPAARMYEEVLKLFFAGKAVANFDMMQEYK
LFAPLFPQVDALLKEAPKGPAMKMVQAIMKSTDDRVNEDKPVTPSFFYAAILWYPLRQRA
EDIAVESGLTLYDAFFAAMGDVMEQQCQTISIPRRFSTPAKDIWQLQLRFERSQGTRAFK
LLEHPKFRAAYDLLLLRGEVEGGNLAKSAAWWQQFVEADETERLTIARSGNKASGNRNRN
TPQRRRRRPSGAKAKAEE