Protein Info for SO0861 in Shewanella oneidensis MR-1

Annotation: conserved hypothetical protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 PF01571: GCV_T" amino acids 22 to 145 (124 residues), 42.7 bits, see alignment E=4.2e-15 TIGR03317: folate-binding protein YgfZ" amino acids 179 to 243 (65 residues), 95.4 bits, see alignment E=7.7e-32 PF21130: YgfZ_barrel" amino acids 235 to 301 (67 residues), 48.3 bits, see alignment E=8.7e-17

Best Hits

Swiss-Prot: 40% identical to YGFZ_VIBPA: tRNA-modifying protein YgfZ (VP2583) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K06980, (no description) (inferred from 100% identity to son:SO_0861)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EIH8 at UniProt or InterPro

Protein Sequence (318 amino acids)

>SO0861 conserved hypothetical protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MTIAVSTPNWDLDASMPPLMLANLSHLGLIKVAGEQGRSFIHGQVTADISSLETNQWRWG
AHCDPKGKMLASFRTFTIKDALLLLMPKDTIEVDLPQLQKYAVFSKATLSNASEEWCLLG
VAGEQANQFVTQHFGEVAQELTLTEHGAILKDADRFILVLQPQAASALIAEHTVFDASAW
QALEIAAGYPNLAPSHANQYVPQMCNLQAINGISFNKGCYMGQETVARMKYRGGNKRALY
ILHGTTSLTINLETSLEIALEDGYRKGGQIIEFVQRGNQVLLTAVLANDTENDAKLRFTE
DEQSSLTVQPLPYSLDEA