Protein Info for SO0846 in Shewanella oneidensis MR-1

Name: napH
Annotation: iron-sulfur cluster-binding protein napH (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 transmembrane" amino acids 36 to 54 (19 residues), see Phobius details amino acids 89 to 118 (30 residues), see Phobius details amino acids 143 to 163 (21 residues), see Phobius details amino acids 170 to 194 (25 residues), see Phobius details TIGR02163: ferredoxin-type protein, NapH/MauN family" amino acids 28 to 280 (253 residues), 382.9 bits, see alignment E=3.7e-119 PF12801: Fer4_5" amino acids 88 to 132 (45 residues), 53.3 bits, see alignment 6.6e-18 amino acids 180 to 222 (43 residues), 31.3 bits, see alignment 4.9e-11 PF13237: Fer4_10" amino acids 226 to 272 (47 residues), 32.1 bits, see alignment 2.6e-11

Best Hits

Swiss-Prot: 53% identical to NAPH_HAEIN: Ferredoxin-type protein NapH (napH) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K02574, ferredoxin-type protein NapH (inferred from 100% identity to son:SO_0846)

MetaCyc: 48% identical to ferredoxin-type protein NapH (Escherichia coli K-12 substr. MG1655)
RXN-18584 [EC: 7.1.1.8]

Predicted SEED Role

"Polyferredoxin NapH (periplasmic nitrate reductase)" in subsystem Nitrate and nitrite ammonification

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.1.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EIJ3 at UniProt or InterPro

Protein Sequence (319 amino acids)

>SO0846 iron-sulfur cluster-binding protein napH (NCBI ptt file) (Shewanella oneidensis MR-1)
MNSKAKARTQNEHAAAIAELGWFGAHKYLLLRRTVQLSLLGLFALGPLLGIWLFKGNLSA
SLLLDTIPFADPLVSLQLLLAGHTPELSLLIGAGLVTAFYALAGGRVFCSWVCPVNLVTD
TASWLRRKLKLPRTSELPRNLRYYLLALVLLLPLLTGSLIWEWVNPVPLVYRAVLFGSLS
GLWILAAIFLLDLFIAERAWCGHLCPTGALFGLVGKWSPIKIVASKASACDNCMDCFAVC
PERQVLKPALKGQSPVISSPDCTQCGRCIDVCAQRVFRYRTRFTGTQTQLSTGGSTNSQS
PSSSSPIRQHIAPKAENDQ