Protein Info for SO0602 in Shewanella oneidensis MR-1

Name: miaA
Annotation: tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01745: IPT" amino acids 8 to 110 (103 residues), 31.2 bits, see alignment E=1.5e-11 TIGR00174: tRNA dimethylallyltransferase" amino acids 9 to 294 (286 residues), 349.7 bits, see alignment E=5.9e-109 PF01715: IPPT" amino acids 43 to 285 (243 residues), 299.4 bits, see alignment E=2.3e-93

Best Hits

Swiss-Prot: 100% identical to MIAA_SHEON: tRNA dimethylallyltransferase (miaA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 100% identity to son:SO_0602)

MetaCyc: 66% identical to tRNA dimethylallyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6274 [EC: 2.5.1.75]

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8CX50 at UniProt or InterPro

Protein Sequence (308 amino acids)

>SO0602 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MNKELQPKVVFLMGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSAEELA
RGPHRLIDIRDPSESYSAADFRADALSEIEQIISMGKTPLLVGGTMMYFKALLEGLSPLP
SADEVIRADIQAEADANGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVYRISGKSLT
ELTMTKSAPLPYNVVQFAIAPRERKVLHELIGQRFRIMLEQGFIDEVAQLKARDDLHLDL
PSMRCVGYRQCWQYLDGEFDHDTMVEKAIAATRQLAKRQLTWLRSWPELNWLESGAEGNL
VTLMRHCR