Protein Info for Rru_A3790 in Rhodospirillum rubrum S1H

Annotation: Glycolate reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 44% identical to GYAR_THEON: Glyoxylate reductase (gyaR) from Thermococcus onnurineus (strain NA1)

KEGG orthology group: K00015, glyoxylate reductase [EC: 1.1.1.26] (inferred from 100% identity to rru:Rru_A3790)

MetaCyc: 42% identical to glyoxylate reductase (Pyrococcus furiosus DSM 3638)
Glyoxylate reductase. [EC: 1.1.1.26]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

photorespiration I (6/9 steps found)
photorespiration III (6/9 steps found)
photorespiration II (6/10 steps found)
L-serine biosynthesis II (1/4 steps found)
L-arabinose degradation IV (3/8 steps found)
D-xylose degradation IV (2/7 steps found)
formaldehyde assimilation I (serine pathway) (6/13 steps found)
superpathway of pentose and pentitol degradation (9/42 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.26, 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.26 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RMR1 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Rru_A3790 Glycolate reductase (NCBI) (Rhodospirillum rubrum S1H)
MPQAKPLVIVTRKLPEAIETRMAELFDARLNVDDHPFTREELCQAVAEADVLVPTVTDRI
DGGVLSHCGESLRLIANFGTGIDHIDVATAHQRGVTVTNTPDVLTEDTADMTMAMILAVP
RRLAEGERMVREGQWLGWSPTHMLGHRIWGKRLGIIGMGRIGRAVARRARGFGMTIHYHN
RRRLHESLEQELDATYWESLDQMLARMDVVSIHCPHTPASYHLLSARRLALLRPEAYVVN
TARGEVIDENALVRMLAKGDLAGAALDVFEYEPAVNPKLVAMNNVLLLPHMGSATLEGRI
DMGEKVLINIKTFVDGHKPPDRVLPTML