Protein Info for Rru_A3690 in Rhodospirillum rubrum S1H

Annotation: Glutathione synthetase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 PF02951: GSH-S_N" amino acids 8 to 126 (119 residues), 144.4 bits, see alignment E=2.5e-46 TIGR01380: glutathione synthase" amino acids 8 to 317 (310 residues), 422.1 bits, see alignment E=5.7e-131 PF02955: GSH-S_ATP" amino acids 130 to 301 (172 residues), 245.4 bits, see alignment E=3.6e-77 PF08443: RimK" amino acids 163 to 313 (151 residues), 46.9 bits, see alignment E=3.8e-16

Best Hits

Swiss-Prot: 62% identical to GSHB_BRUME: Glutathione synthetase (gshB) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 100% identity to rru:Rru_A3690)

MetaCyc: 46% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RN11 at UniProt or InterPro

Protein Sequence (318 amino acids)

>Rru_A3690 Glutathione synthetase (NCBI) (Rhodospirillum rubrum S1H)
MTQSSTTLRVAIQMDPMAAIDINGDSTFALALEAQARGHRLWHYLAQDLVFRDGRVTAKA
HPLSVRREAGHHFSFGAPETIDLGSDVEVVLMRQDPPFDMAYITATHLLDHIHPKTLVVN
DPTEVRNAPEKLFVTRFPELMPPTLITLDRAAIAAFRAEWGDIIVKPLYGNGGAGVFHIA
PGDENLNALLEMFAQRSREPVMVQRYLPEIRQGDKRVILIDGKAAGAVNRVPAAGEARSN
MHIGGRPEKTPLTARDLEICEAIGPILREKGLLFVGIDVIGDWLTEINVTSPTGIQEIDR
FDGVCLEADLWNAIEAKL