Protein Info for Rru_A3624 in Rhodospirillum rubrum S1H

Annotation: tRNA modification GTPase TrmE (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 PF10396: TrmE_N" amino acids 5 to 132 (128 residues), 115.9 bits, see alignment E=2.5e-37 TIGR00450: tRNA modification GTPase TrmE" amino acids 10 to 455 (446 residues), 259.6 bits, see alignment E=6.1e-81 PF12631: MnmE_helical" amino acids 135 to 452 (318 residues), 168.8 bits, see alignment E=3.7e-53 PF01926: MMR_HSR1" amino acids 230 to 320 (91 residues), 76.6 bits, see alignment E=3.4e-25 PF02421: FeoB_N" amino acids 230 to 318 (89 residues), 30.2 bits, see alignment E=6e-11 TIGR00231: small GTP-binding protein domain" amino acids 230 to 317 (88 residues), 52.3 bits, see alignment E=5.8e-18

Best Hits

Swiss-Prot: 100% identical to MNME_RHORT: tRNA modification GTPase MnmE (mnmE) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 100% identity to rru:Rru_A3624)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RN77 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Rru_A3624 tRNA modification GTPase TrmE (NCBI) (Rhodospirillum rubrum S1H)
MTVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPT
GPGPEEGGEVLDDALVLWFPAPASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEA
GEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQADGALVRLYEGWRKTGIGLLA
HLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVG
KSSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRR
ARARLAAADLTLAVSDGTVENGADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALP
VSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSALMEARAALRRAAEAPLAELV
AEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK