Protein Info for Rru_A3575 in Rhodospirillum rubrum S1H

Annotation: Acetate--CoA ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 20 to 641 (622 residues), 1056.1 bits, E=0
• PF16177: ACAS_N
  • amino acids 24 to 81 (58 residues), 75 bits, 4.8e-25
• PF00501: AMP-binding
  • amino acids 83 to 467 (385 residues), 308 bits, E=1.3e-95
• PF13193: AMP-binding_C
  • amino acids 529 to 607 (79 residues), 92.6 bits, E=3.5e-30

Best Hits

Swiss-Prot: 76% identical to ACSA_PARL1: Acetyl-coenzyme A synthetase (acsA) from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to rru:Rru_A3575)

MetaCyc: 68% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• acrylate degradation II (3/3 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• 2-methylcitrate cycle II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• chitin deacetylation (1/4 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (2/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See Q2RNC6 at UniProt or InterPro

Protein Sequence (646 amino acids)

>Rru_A3575 Acetate--CoA ligase (NCBI) (Rhodospirillum rubrum S1H)
MSESVLVPVPEAIAKAALVNEAQYLDLYKSSIADPDAFWAEHGKRIDWIKPFTAVKDVSF
DPHDVHIRWFHDGTLNVSVNCLDRHLETRGDQTAILWEGDDPKDDARITYRDLYERVCKL
ANAFRELGVKKGDRVCIYLPMIPEAAVAMLACARIGAVHSIVFGGFSPDSLANRIQDSEC
TLVITSDEGLRGGRKVALKKNVDTALEHCPSVKTVVVVKRTGGAIDTVEGRDVWYHEITA
NQPAECQPEEMNAEDPLFILYTSGSTGKPKGVLHTSGGYLVYVSMTHEYVFDYKDGEVYW
CTADVGWVTGHSYIVYGPLANGAISLMFEGVPNYPDVSRFWQVVEKHKVNIFYTAPTAIR
ALMREGEEPVKKHDRSSLRILGSVGEPINPEAWTWYHRVVGEDRCPIVDTWWQTETGGIL
ITPLPGATKLKPGSATLPFFGVEPVLVDAEGNELSGATEGNLCIARSWPGQMRTVYGDHE
RFVQTYFSTYKGYYFTGDGCRRDEDGYYWITGRVDDVINVSGHRMGTAEIESALVAHPKV
AEAAVVGFPHDIKGQGIYAYVTLVAGEKETDALRKELVAWVRKDIGPIASPDIIQWAPGL
PKTRSGKIMRRILRKIAENEFGALGDTSTLADPTVVEDLIANRPQA