Protein Info for Rru_A3555 in Rhodospirillum rubrum S1H

Annotation: Heat shock protein Hsp70 (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 TIGR02350: chaperone protein DnaK" amino acids 3 to 598 (596 residues), 994.7 bits, see alignment E=6.6e-304 PF00012: HSP70" amino acids 4 to 598 (595 residues), 929.9 bits, see alignment E=5.4e-284 PF06723: MreB_Mbl" amino acids 125 to 372 (248 residues), 60.9 bits, see alignment E=1e-20

Best Hits

Swiss-Prot: 100% identical to DNAK_RHORT: Chaperone protein DnaK (dnaK) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to rru:Rru_A3555)

MetaCyc: 67% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNE6 at UniProt or InterPro

Protein Sequence (639 amino acids)

>Rru_A3555 Heat shock protein Hsp70 (NCBI) (Rhodospirillum rubrum S1H)
MSKVIGIDLGTTNSCVAVMDGKDVRVIENAEGARTTPSQVAFTESGERLVGQPAKRQAVT
NPENTLFAIKRLIGRRYSDPTVEKDKGLVPYRIVKGDNGDAWVQTRDEKYAPSQLSAFIL
QKMKETAEAHLGEPVTQAVITVPAYFNDSQRQATKDAGKIAGLEVLRIINEPTAAALAYG
MDKKNTGTIAVFDLGGGTFDVSVLEIGDGVFEVKSTNGDTFLGGEDFDARIIDYLASEFK
KEQGIDLRTDRLALQRLKEAAEKAKIELSSSMQTEVNLPFITADQAGPKHLNIKLTRAKL
EALVDDLVQRTVEPCRKALADAGIKASEIDEVILVGGMTRMPKVQQVVKDFFGREPHKGV
NPDEVVAMGAAIQGGVLKGDVKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTRKSQT
FSTAEDSQTAVTIRVFQGEREMAADNKMLGQFDLVGLPSAPRGVPQIEVTFDIDANGIVN
VSAKDKATGKEQAIRIQASGGLSDNDIERMVKEAELNAEADRKRKEAVEARNHADGLIHA
TEKNLKEYGDKIPAEDKAKVEGDLTALKAVLDSEDAESIKAKTDALMQSAMKLGEAAYSA
GQSAEGAPHAAGAEASAQSRTDDGVVDADFEEVDEKKGH