Protein Info for Rru_A3548 in Rhodospirillum rubrum S1H
Annotation: Magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to BCHE_RUBGE: Anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase (bchE) from Rubrivivax gelatinosus
KEGG orthology group: K04034, anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase [EC: 4.-.-.-] (inferred from 100% identity to rru:Rru_A3548)MetaCyc: 67% identical to anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase (Rhodobacter capsulatus)
RXN-13852 [EC: 1.21.98.3]
Predicted SEED Role
"Mg-protoporphyrin IX monomethyl ester oxidative cyclase (anaerobic) (EC 1.14.13.81)" in subsystem Chlorophyll Biosynthesis (EC 1.14.13.81)
MetaCyc Pathways
- 3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent) (7/9 steps found)
- 3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent) (6/9 steps found)
- 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) (6/9 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (13/26 steps found)
KEGG Metabolic Maps
- Biosynthesis of type II polyketide backbone
- Photosynthesis - antenna proteins
- Porphyrin and chlorophyll metabolism
- Vitamin B6 metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.-.-.-
Use Curated BLAST to search for 1.14.13.81 or 1.21.98.3 or 4.-.-.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RNF3 at UniProt or InterPro
Protein Sequence (535 amino acids)
>Rru_A3548 Magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (NCBI) (Rhodospirillum rubrum S1H) MRILFVHPNYHSGGAEVAGKWSPAWVAYLAGYLKAGGYSDIRFVDAMTDDMTEEELRAIF AAEQPDVIGTTAVTPAIYKAERVLEIAREECPNAVTILGGIHATFMFQQVLVEAPWIDTV VRGEGEAVTLNLIRAIDEGRWPADRASIKGIAYLEDTKVVATPAEPPIRDVDSIIPDWGV LEWKKYIYIPLGVPVATPNMARGCPFTCSFCSQWKFWRDYRVRDPKKVVDEIEILVRQHG VGFFILADEEPTINRNKFVEFCEELIRRDLGVMWGINTRVTDIMRDEDLLPLYRKAGLIH ISLGTEAAAQLNLDIFHKETTVAQNKRAIELLRKHGIVTEAQFIVGLENETAETLEETYK MCMDWNPDMANWSMFTPWPFSDLFHELGDKVEIFDFEKYNFVTPIMKPNAMDRAELLDRV MHNYRRFYMRKSFLGYPWVLNRVRRRYLLGCLKAFLLSAFQRKFYDLGRAGYWGPQSKKK VDFHFDHSRTLVKPQEDNWKNAPKHKRPAAAMKACGGGDEQLAESLTPPPVKVKI