Protein Info for Rru_A3539 in Rhodospirillum rubrum S1H

Annotation: GlnB (protein PII) uridylyltransferase, GlnD (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 936 transmembrane" amino acids 608 to 626 (19 residues), see Phobius details TIGR01693: protein-P-II uridylyltransferase" amino acids 58 to 909 (852 residues), 938.4 bits, see alignment E=2.1e-286 PF01909: NTP_transf_2" amino acids 105 to 183 (79 residues), 22.9 bits, see alignment E=2.7e-08 PF08335: GlnD_UR_UTase" amino acids 210 to 348 (139 residues), 163.9 bits, see alignment E=7.2e-52 PF01966: HD" amino acids 490 to 583 (94 residues), 35 bits, see alignment E=4.9e-12 PF24931: ACT_ACR9_3rd" amino acids 828 to 913 (86 residues), 43.6 bits, see alignment E=7.8e-15

Best Hits

Swiss-Prot: 100% identical to GLND_RHORT: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to rru:Rru_A3539)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNG2 at UniProt or InterPro

Protein Sequence (936 amino acids)

>Rru_A3539 GlnB (protein PII) uridylyltransferase, GlnD (NCBI) (Rhodospirillum rubrum S1H)
MTIPRIRQPRAVIDRKALTVVLEDLAATVTDNRERRARLLAVLKGALGDGRAEVRRRFLE
EKGTGAAVFAENSHLMDQIIRLLFDFTTTHVYPRANRTIGEQMTVLAVGGYGRGEMSPQS
DVDLLFLLPYKATPLHEQVVEYMLYTLWDMGLKVGHATRSIEECIRQARGDLTIRTAMLE
TRYLWGDRALYGQLKTKFWTGVVTGTGPDFVEAKLAERDERHLRMGDSRYVLEPNIKDGK
GGLRDLHTLLWIARYIYGVSDMRELVELGVLSADAATKFGRARAFLWTVRCHLHYLADRP
EERLTFDVQPAIAARMGYTDRNSGRGVERFMKHYFLMAKTVGDLTRIFCAVLEDQQKRRP
ILSIATLLMRKRNLGDFVLDGGRLAVAGRGAFREHPLQLISLFKVAHDHGLDIHPDTLRL
VTEHLPTVTLLRNDAKANALFMEILTSRKDPELALRQLSESGVLARFIPDFGRVTAQMQF
DMYHVYTTDEHTIRAIGLLHRLETGALRDRMPAAADAVHKVQSRRALYLAVLLHDIAKGR
GGDHSILGAEVAMRLGPRLGMSEEETETVAWLVRHHLDMSRTAFKRDLDDIKTILDFTGL
VQSVERLHLLLALTTVDILAVGPAVWNNWKSSLLRELYTHSKDVLTSGFQAEARDKRVAH
KREELAAALADWPQASRERYLDLHYPAYWLTFDSATHLRHARMLRRARDAGLTVAVEVLP
DPERAVSEVLVATDDHPGLFSKIAGAMALAGVNILDAKITTMSDGGALDIFTVQTLEGHA
IEKEERIARLAKTVRDVLTGDLPLEKALRRQPPRLPERTRHLTVPPRVIVDNQASKTHTV
IEINGRDRPGFLYAVTRALTDVAVQISSARVSTYGERVVDSFYVKDVFGMKIVHRAKLAQ
IREALEAAITQTVPRKVEEGAEQGAEKADAGEIVAA