Protein Info for Rru_A3524 in Rhodospirillum rubrum S1H

Annotation: phenylalanyl-tRNA synthetase, beta subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 799 (799 residues), 670.3 bits, see alignment E=2.1e-205 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 77 bits, see alignment E=2.5e-25 PF03483: B3_4" amino acids 215 to 388 (174 residues), 200.8 bits, see alignment E=3.5e-63 PF03484: B5" amino acids 407 to 473 (67 residues), 66.5 bits, see alignment E=4.8e-22 PF17759: tRNA_synthFbeta" amino acids 481 to 686 (206 residues), 152.4 bits, see alignment E=3.1e-48 PF03147: FDX-ACB" amino acids 706 to 799 (94 residues), 81.7 bits, see alignment E=1.1e-26

Best Hits

Swiss-Prot: 100% identical to SYFB_RHORT: Phenylalanine--tRNA ligase beta subunit (pheT) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to rru:Rru_A3524)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNH7 at UniProt or InterPro

Protein Sequence (800 amino acids)

>Rru_A3524 phenylalanyl-tRNA synthetase, beta subunit (NCBI) (Rhodospirillum rubrum S1H)
MKFTIGWLKDHLDTQASVADIAEKLTALGLEVDAIEDPTAALAPFVVGHVIEAGPHPDAD
RLKLCRVDSGTDILQIVCGAPNARTGLKVALALPGAVIPATGDVLKKGKIRGIESQGMMC
STRELGLGEDHDGIIELPADTPVGVPLVDVLALDPVIDIAITPNRADALGVRGIARDLAA
GGLGGVIADTLTAIPGRFPSPIGVAIDPETLASGACSHYVGRYFRGVRNGESPAWLKDRL
NAIGVRPISLLVDITNYVTFDRARPLHVFDADKLTGTTITARPARQGEILRALDGRSYTL
DAPVVAIADGAGPQGLGGIMGGEDTGVDENTVNVFLESALFDPANTAAAGRFLGIDSDAR
YRFERGVDPESCLLGAELATRMILDLCGGEASELVIAGAPPVWRRTIALRPARVARLGGV
AVERAEMVAKLRALGCTVEDDGAEALRVDPPSWRVDIGAEHDLIEEVVRLHGFDLVPAVP
LPRDPMPKAVLTPGQRRMITVKRTLATRGMLEAVTWSFLPRAVAKGFGGGQDALVLANPI
SSDLDAMRPSILPNLIAAAGRNAARGYGDLGLFEVGPRFHGGEPGQQTLVAAGLRAGKIA
PRHWSRPARDADLYDIKADVLAAIEAAGAPTASLQVSADAPAWFHPGRSGQIRLGRTVLA
AFGEIHPAALALLDVKGPMVGFELDLDALPLPKGRPSKTRPPLTLSPFQPVARDFAFVVE
AGVAGDAVIKAARGADKALITDVVIFDVYQGDRLEAGRKSLALSVTLQPTDHTLSDEEIE
AVSARIVGAVTKLTGGSLRS