Protein Info for Rru_A3461 in Rhodospirillum rubrum S1H

Annotation: Carbohydrate kinase, FGGY (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 PF00370: FGGY_N" amino acids 16 to 259 (244 residues), 211.7 bits, see alignment E=1.4e-66 PF02782: FGGY_C" amino acids 272 to 456 (185 residues), 127.8 bits, see alignment E=4.7e-41

Best Hits

Swiss-Prot: 55% identical to GLPK_RHOCS: Glycerol kinase (glpK) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to rru:Rru_A3461)

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNP0 at UniProt or InterPro

Protein Sequence (505 amino acids)

>Rru_A3461 Carbohydrate kinase, FGGY (NCBI) (Rhodospirillum rubrum S1H)
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPE
DIWLDVLTAWRQVLEGSGADHVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARC
RELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGARAAAEAGHLAFGTVDSFLLWR
LTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYR
LDGEVTYALEGTVFSAGAAIGWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGA
PYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLEAMIADGLPRPAVLRVEGAMS
ANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
IDPAYRRQLLDGWRKAVGRVQESAP