Protein Info for Rru_A3423 in Rhodospirillum rubrum S1H

Annotation: Periplasmic Sensor Signal Transduction Histidine Kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 32 to 53 (22 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details PF13755: Sensor_TM1" amino acids 26 to 80 (55 residues), 46.2 bits, see alignment 7.7e-16 PF13756: Stimulus_sens_1" amino acids 101 to 212 (112 residues), 105 bits, see alignment E=7.7e-34 PF00672: HAMP" amino acids 273 to 326 (54 residues), 35.5 bits, see alignment 2.6e-12 PF00512: HisKA" amino acids 334 to 397 (64 residues), 56.5 bits, see alignment E=5.7e-19 PF02518: HATPase_c" amino acids 446 to 559 (114 residues), 87.6 bits, see alignment E=1.9e-28

Best Hits

KEGG orthology group: K14980, two-component system, OmpR family, sensor histidine kinase ChvG [EC: 2.7.13.3] (inferred from 100% identity to rru:Rru_A3423)

Predicted SEED Role

"Sensor histidine kinase ChvG (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNS8 at UniProt or InterPro

Protein Sequence (564 amino acids)

>Rru_A3423 Periplasmic Sensor Signal Transduction Histidine Kinase (NCBI) (Rhodospirillum rubrum S1H)
MRADPGDRPDSTQEPKRRRHRRPRGLLRSPLSRRILAVNLIAPILLAGGFLYLDTYEDAL
TASTFQAMRTEADLIAAAIGEGAINLEVSVEGPVVRATHGINPEMSRQMVRRLANLAEVR
ARLFDTSGTLVADSRRLVGYGGMVQVMDLPPPVLPDNGVGLLRRFYDAIIHPSSDLAKLQ
PYIERPNQSAEDYPEVLRALTEGESGFFMRVRPTRDRVLSVAVPVQYYKQVVGAVMVSRG
AEEIDETLFEMRLGVIEIFAFTLALTILLSFYIAGTIVRPLLRLAGAADRVRHGKAGRHE
AIPDFSNRRDELGDLSAALREMTEVLWARMDAIERFAADVAHEIKNPLTSLRSAVETVAR
IDDPNQQKRLMAVIVDDVQRLNRLITDISDASRLDAELSRAEMESVEIRPMLEMLAEVHA
AEAEQRGVTIKVMGDAGDALGIEGLEGRLVQVFRNLIGNAVSFSPPGGTITLRAWRTKGK
VVITVADDGPGIPPGKEADIFKRFYSERPREEKFGTHSGLGLSISQQIVETHRGTIRAEN
RRDGEGKIVGAMFTVELPSPTPGK