Protein Info for Rru_A3419 in Rhodospirillum rubrum S1H

Annotation: Phosphoenolpyruvate carboxykinase (GTP) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 PF17297: PEPCK_N" amino acids 18 to 239 (222 residues), 350.1 bits, see alignment E=4.8e-109 PF00821: PEPCK_GTP" amino acids 243 to 602 (360 residues), 544.1 bits, see alignment E=1.3e-167

Best Hits

Swiss-Prot: 72% identical to PCKG_GEOSL: Phosphoenolpyruvate carboxykinase [GTP] (pckG) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

KEGG orthology group: K01596, phosphoenolpyruvate carboxykinase (GTP) [EC: 4.1.1.32] (inferred from 100% identity to rru:Rru_A3419)

MetaCyc: 55% identical to phosphoenolpyruvate carboxykinase [GTP], mitochondrial (Homo sapiens)
Phosphoenolpyruvate carboxykinase (GTP). [EC: 4.1.1.32]; 4.1.1.32 [EC: 4.1.1.32]

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [GTP] (EC 4.1.1.32)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.32)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNT2 at UniProt or InterPro

Protein Sequence (620 amino acids)

>Rru_A3419 Phosphoenolpyruvate carboxykinase (GTP) (NCBI) (Rhodospirillum rubrum S1H)
MLEEEMSVKPTRNKKLLDWVEDMTALCKPDRVYWCDGSQEEYDRLCQAMVDDGTFIRLNP
ELRPNSFLARSDPADVARVEDRTFICAATADEAGPTNNWMAPDEMRAKLAGLFDGCMKGR
TLYVIPFSMGPLGSHIAQIGVEISDSPYVAANMRIMTRMGSKVLDVLGEDGDFVPAMHSV
GKPLAPGEVDKGWPCNPENRYIVHFPDTREIWSFGSGYGGNALLGKKCFALRIASVMARD
EGWLAEHMLILGVKSPTGEKRYVGGAFPSACGKTNFAMLIPPEAYKGWEVTTIGDDIAWI
KPGADGRLYAINPEAGYFGVAPGTGVHTNPNAMIACAKNSIFTNVALTDDGDVWWEGMTE
KKPAHAIDWKGQDWTPDCGRPAAHPNARFTAPANQAPSIDPDWENPDGVPISAFIFGGRV
ATNFPLAFQSFDWTHGVYLAATMGSEATAAADGQSGIRRDPMAMLPFCGYNMADYWQHWL
DMEDKVGADKLPRIFRVNWFRKDENGKFIWPGFGENMRALEWIIGRCEGTAGAVESPIGF
VPRREDLRWDGIVGFDVSRFYQIMAIHKDVARKEAETQDVFFAKFGDRMPKALLDQRTAL
LKRLEDAPEVWDLPRAEAAQ