Protein Info for Rru_A3365 in Rhodospirillum rubrum S1H

Annotation: Small GTP-binding protein domain (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 26 to 205 (180 residues), 184.5 bits, E=3.1e-58
• TIGR01393: elongation factor 4
  • amino acids 26 to 618 (593 residues), 948.9 bits, E=1e-289
• TIGR00231: small GTP-binding protein domain
  • amino acids 28 to 198 (171 residues), 66.7 bits, E=2.1e-22
• PF00679: EFG_C
  • amino acids 424 to 507 (84 residues), 74.7 bits, E=9.6e-25
• PF06421: LepA_C
  • amino acids 511 to 616 (106 residues), 166.6 bits, E=3.3e-53

Best Hits

Swiss-Prot: 100% identical to LEPA_RHORT: Elongation factor 4 (lepA) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to rru:Rru_A3365)

MetaCyc: 58% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (11/14 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (5/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (5/8 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q2RNY6 at UniProt or InterPro

Protein Sequence (621 amino acids)

>Rru_A3365 Small GTP-binding protein domain (NCBI) (Rhodospirillum rubrum S1H)
MALWSRPSGRLPRRSSPRREMSDLSHIRNFAIIAHIDHGKSTLADRLIQTCGGVEARDMK
EQLLDSMDLERERGITIKAQTVRLHYTAKSDGRTYQLNLMDTPGHVDFAYEVSRSLAACE
GSLLVVDASQGVEAQTLANVYQAIDAGHEIIPVLNKIDLPAAEPERIKQQIEDVIGLDTS
DAVGISAKTGLNIDQVLEALVLRLPPPTGDVDAPTQALLVDSWYDSYLGVVILVRVRHGV
LRKGMKMLMMATGAVHPIDKVGVFTPKMLETDSLSAGEMGFVMAGIKSVADCQIGDTITD
DRAPAAEALPGFKASIPVVWCGLFPVDSSDFEVLRESLAKLRLNDSSFEFQAESSAALGF
GFRCGFLGLLHMEIIQERLEREFGLDLITTAPSVVYRMHMTDGTLLELHNPADMPDPVRL
DFVEEPWVKATIMVPDEYLGQILALCTERRGIQMDLTYAGSRAMAVYKLPLNEIVFDFYD
RLKSISRGYASFDYEVADYAESDLVKVSILVNNEPVDALAFIAHRTQADFRGRQICGRLK
DLIPRHLFKVPIQAAIGGRVIARETIAAMRKDVTAKCYGGDITRKKKLLEKQKEGKKKMR
QFGKVEIPQSAFINALRMSDD