Protein Info for Rru_A3329 in Rhodospirillum rubrum S1H

Annotation: hypothetical protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 PF21016: RlmN_N" amino acids 48 to 107 (60 residues), 75 bits, see alignment E=3.1e-25 TIGR00048: 23S rRNA (adenine(2503)-C(2))-methyltransferase" amino acids 48 to 398 (351 residues), 413.1 bits, see alignment E=4.5e-128 PF04055: Radical_SAM" amino acids 151 to 321 (171 residues), 53.9 bits, see alignment E=2.6e-18

Best Hits

Swiss-Prot: 100% identical to RLMN_RHORT: Dual-specificity RNA methyltransferase RlmN (rlmN) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 100% identity to rru:Rru_A3329)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RP22 at UniProt or InterPro

Protein Sequence (428 amino acids)

>Rru_A3329 hypothetical protein (NCBI) (Rhodospirillum rubrum S1H)
MPLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAA
LLRDMGEKPFRAKQLWHWVYHRGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSED
GTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTCRFCHTGTQLLVRNLTAHEIV
GQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRR
ITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGA
SNARRITFEYVMLKGVNDSEADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIER
FANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASLHKARLAAGVADDHAPAAAPL
ADTPGAVA