Protein Info for Rru_A3175 in Rhodospirillum rubrum S1H
Annotation: Glutamyl-tRNA(Gln) amidotransferase B subunit (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GATB_RHORT: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (gatB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
KEGG orthology group: K02434, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to rru:Rru_A3175)MetaCyc: 45% identical to glutamyl-tRNAGln amidotransferase subunit B (Bacillus subtilis)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RPH5 at UniProt or InterPro
Protein Sequence (485 amino acids)
>Rru_A3175 Glutamyl-tRNA(Gln) amidotransferase B subunit (NCBI) (Rhodospirillum rubrum S1H) MTYLIKGETATWEVVVGLEVHAQVVSQSKLFSGSSTRFGAPANSQVSLVDAAMPGMLPVI NGLCVEQAVRTGLGLRAKINKRSIFARKNYFYADLPQGYQISQYEQPIVGEGKVVLDMPD GSSREVGIERLHLEQDAGKSMHDQHPTKSLIDLNRSGVALMEIVSKPDMREPEEAGAYLR KLRSIMRYLGTCDGNMEEGSMRADVNVSVRPIGTTDLRTRCEIKNVNSVRFVMQAIEAEA HRQVEVWESGGEVRQETRLFDPAKGETRSMRSKEHAHDYRYFPDPDLLPLEFDDAFVEKV RRSLPELPDDKKNRFIADFGLTPYDASVLVVDRENAAFFEKVAEGRDGKTAANWVINNLF GALAKAGKGVADSPVSAENLGKLLDLLKDEVINGRIAKDVFEIMMETGEDPDAIVEDKGL RQVTDTSAIEAAVDAVIAANPEKADDVRGGKEKLLGWFVGQVMKATQGKANPAMLNDILR AKLLG