Protein Info for Rru_A3121 in Rhodospirillum rubrum S1H

Annotation: Asparagine synthase, glutamine-hydrolyzing (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03108: exosortase A system-associated amidotransferase 1
  • amino acids 1 to 628 (628 residues), 1070.4 bits, E=0
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 562 (561 residues), 471.1 bits, E=5.8e-145
• PF13522: GATase_6
  • amino acids 33 to 162 (130 residues), 138.2 bits, E=3.4e-44
• PF13537: GATase_7
  • amino acids 48 to 167 (120 residues), 154.3 bits, E=2.8e-49
• PF00733: Asn_synthase
  • amino acids 244 to 620 (377 residues), 324.9 bits, E=1.9e-100

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to rru:Rru_A3121)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) AsnH" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See Q2RPM9 at UniProt or InterPro

Protein Sequence (641 amino acids)

>Rru_A3121 Asparagine synthase, glutamine-hydrolyzing (NCBI) (Rhodospirillum rubrum S1H)
MCGLVGIYHPTASRPVDRALVEAMNQRQFHRGPDDGGVFIGPGIGLGHRRLSIVDLGGGR
QPMTNDGGGVVLVYNGELYNNAELVAELKAAGHVFRTASDTETVIHAWEEWGEDSVRHLR
GMFAYALWDDRSQTLFLARDRLGIKPLHYTCLADGRVMFGSELKSLTGVEDIPKVVDPLA
IEDFLAYGYVPDPRSIFKDILKLEAGHTLTFRRGEAPGRPHCYWDVSFTTPSRPAKTDDL
CAEVIDRLDEAVRCHLVADVPVGAFLSGGVDSSAMVALMAGATDRPVETCSIAFSEARFD
ESAYAKTVAERYGTHHRVHTLPAQALSEVGPLTAIYDEPFADSSALPTLKVCQAARETVT
VALSGDGGDELFAGYRRYRFHTAEEAVRRILPQAARGPLFGALGRVYPKLDWAPQVLRAK
STFQALGRDRVEGYFNAVSVIPDGLRRGLYSKTMRAELAGYGASEVLRRHMTRAPADHPL
SIAQYVDLKTWLPGDILTKVDRASMATSLEVRVPMLDHRFVEWTAGLSPDLKLSGGTGKY
LLKRALRPLLPDEVLYRRKMGFSIPLTDWLRGPWSGRTRALAGRSALVTSGLFDGAAVAK
LVEQHLGGNWDHGAALYALIVLDDFLTAHVGAACLPARMSA