Protein Info for Rru_A3108 in Rhodospirillum rubrum S1H

Annotation: NAD-dependent epimerase/dehydratase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 18 to 218 (201 residues), 35.5 bits, E=1.8e-12
• PF01370: Epimerase
  • amino acids 19 to 241 (223 residues), 185.2 bits, E=4.2e-58
• PF02719: Polysacc_synt_2
  • amino acids 19 to 273 (255 residues), 49.4 bits, E=1.2e-16
• PF01073: 3Beta_HSD
  • amino acids 20 to 234 (215 residues), 68.2 bits, E=1.9e-22
• PF16363: GDP_Man_Dehyd
  • amino acids 20 to 267 (248 residues), 130.2 bits, E=3.7e-41

Best Hits

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to rru:Rru_A3108)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Sequence Analysis Tools

See Q2RPP2 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Rru_A3108 NAD-dependent epimerase/dehydratase (NCBI) (Rhodospirillum rubrum S1H)
MTALTEIDARHAAWRGRRVLVTGGAGFIGGHLCRRLVGLGAEVVVLDDLSTGRRDTVPRG
VRLIVGSVTDPALVRKALQGTEGCFHLAAIASVPLSVSALVDCHAVNQTGTLRLIEGLRD
NGGGRLVYASSSAVFGDPVALPLTMASPTRPISPYGVDKLACEAHARVAGGLYGLKSFGL
RFFNVYGEGQSDDSPYSGVIALFNRKLRDGQPITVFGDGSQSRDFVYVGDVIEGLLAAWN
EASVRGPVETVGTGCPTTVMDLARTIMEVHGRSVPVVHAPPREADIEHSYGKADFLARIL
PKPAVALRAGLARTLSISPPAATLAPTP