Protein Info for Rru_A3051 in Rhodospirillum rubrum S1H

Annotation: Glycine cleavage system T protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00528: glycine cleavage system T protein
  • amino acids 13 to 369 (357 residues), 347.7 bits, E=3.3e-108
• PF01571: GCV_T
  • amino acids 17 to 262 (246 residues), 290 bits, E=1.5e-90
• PF08669: GCV_T_C
  • amino acids 290 to 368 (79 residues), 77.3 bits, E=6.7e-26

Best Hits

Swiss-Prot: 47% identical to GCST_DICDI: Aminomethyltransferase, mitochondrial (gcvT) from Dictyostelium discoideum

KEGG orthology group: K00605, aminomethyltransferase [EC: 2.1.2.10] (inferred from 100% identity to rru:Rru_A3051)

MetaCyc: 52% identical to aminomethyltransferase (Gallus gallus)
Aminomethyltransferase. [EC: 1.4.1.27, 2.1.2.10]; 1.4.1.27 [EC: 1.4.1.27, 2.1.2.10]

Predicted SEED Role

"Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 2.1.2.10)

MetaCyc Pathways

• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• glycine degradation (2/3 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• folate transformations II (plants) (7/11 steps found)
• photorespiration II (6/10 steps found)
• folate transformations I (8/13 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Nitrogen metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 1.4.1.27 or 2.1.2.10

Sequence Analysis Tools

See Q2RPU9 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Rru_A3051 Glycine cleavage system T protein (NCBI) (Rhodospirillum rubrum S1H)
MEPHLSDPTVSLLTTPLHALHLERGARMVPFAGYDMPVQYPMGVLAEHLHTRASAGLFDV
SHMGQARLVGPQRIAALEALVPGDLEILKEGRQRYTVLTNDQGGILDDLMVTKRADDLFL
VVNAACKDADLDHIEAHLAGFDARLERLPDTALLALQGPLAVSVLAGLDARAAEMGFMSG
RWLSLCGVDCFVTRSGYTGEDGFEISVPAEAALDLAQTLIANEAVALIGLGARDSLRLEA
GLCLYGSDIDTTTTPVEAGLSWIIGKRRRAEGGFPGASAIQQDLAQGPKRCRVGLRPEGK
APVRAHSAILGPQGEVVGEVTSGGFSPSLSAPIAMGMVPAELAAPGTAVSLVVRGKALPA
HVVEMPFVAHRYHKA