Protein Info for Rru_A2966 in Rhodospirillum rubrum S1H

Annotation: Isoleucyl-tRNA synthetase, class Ia (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 TIGR00392: isoleucine--tRNA ligase" amino acids 16 to 845 (830 residues), 817.5 bits, see alignment E=7.3e-250 PF00133: tRNA-synt_1" amino acids 29 to 649 (621 residues), 430.9 bits, see alignment E=9.7e-133 PF09334: tRNA-synt_1g" amino acids 59 to 205 (147 residues), 37.5 bits, see alignment E=2.5e-13 PF08264: Anticodon_1" amino acids 694 to 845 (152 residues), 92.1 bits, see alignment E=6.5e-30 PF06827: zf-FPG_IleRS" amino acids 905 to 932 (28 residues), 25.4 bits, see alignment (E = 2e-09)

Best Hits

Swiss-Prot: 56% identical to SYI_CAUVC: Isoleucine--tRNA ligase (ileS) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 100% identity to rru:Rru_A2966)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RQ34 at UniProt or InterPro

Protein Sequence (940 amino acids)

>Rru_A2966 Isoleucyl-tRNA synthetase, class Ia (NCBI) (Rhodospirillum rubrum S1H)
MTADYKSTVFLPTTDFPMKAGLAEKEPGILARWEKLDIYARLRERSKGREQFVLHDGPPY
ANGHLHNGHALNKILKDVITRSQQMLGKDANYVPGWDCHGLPIEWKIEERYRAQGRRKDE
IDVIEFRRECRQFAEEWIAIQREEFKRLGITGDWKRPYTTMAFAAEAQIVRELGKFLMAG
DLYKGAKPVLWSVVEKTALADAEVEYQDHTSTTIWIRFPVIRTPVTALEGASVVIWTTTP
WTMPGNRAVAYGPGMDYVVFRVTATAEGSLARAGERIAVCADLLDDVLKQAKITEIEEIA
HVKGADLAGTVCRHPWAGKGYDFEVPLLSGLFVTTEQGTGFVHMAPGHGEDDYFLCLANG
IAPPDTVDGDGLYMDHVPGFAGKHVFKVAPDIVAAMIDAGALLAQGSLTHSYPHSWRSKA
PLIFRNTPQWFISMEKNGLRDKALAAIDETRWVPPQGRNRIRAMIESRPDWCVSRQRSWG
VPIAIFVDKRDGLPLRDAAVTERIAQAFGAEGADAWFTGDPRRFLGAEHDPENFEPVRDI
VEVWFDSGSTHAFVLEARDDLKWPADLYLEGSDQHRGWFHTSLLESCGTRGRAPYDAVLT
HGFLLDEKGDKLSKSKGNAESPQKVVSSVGADIMRLWVVSSDYTGDIRFGPEILKQTTDT
YRRLRNTLRFLLGALAGFDKAERIDDIAAMPELERWVLHRLSEIDVKVRGCCDAFSFHEM
FQELHGFCAVDLSAFYLDIRKDALYCDGAGSLRRRACRTVIDTVFDCLVKWLAPFVCFTA
EEAWLTRFPSEDDSVHFQSFPTVPGDWRDDALAERWTKVRRLRRVITGALEVARANKTIG
ASLQAAPVVHAPAELLNACAGLDMAEISITSDITLTDSADQPIPEGAYTLDEVAGVGVVV
ALAEGEKCQRCWEILPDVGTHAHEGLCGRCDEVVSSLTAG