Protein Info for Rru_A2955 in Rhodospirillum rubrum S1H

Annotation: Glycine--tRNA ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 719 (717 residues), 575 bits, see alignment E=1.7e-176 PF02092: tRNA_synt_2f" amino acids 4 to 576 (573 residues), 626.4 bits, see alignment E=5.1e-192 PF05746: DALR_1" amino acids 607 to 712 (106 residues), 27 bits, see alignment E=4.2e-10

Best Hits

Swiss-Prot: 100% identical to SYGB_RHORT: Glycine--tRNA ligase beta subunit (glyS) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to rru:Rru_A2955)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RQ43 at UniProt or InterPro

Protein Sequence (721 amino acids)

>Rru_A2955 Glycine--tRNA ligase (NCBI) (Rhodospirillum rubrum S1H)
MAELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLP
VAQPDVHEERRGPKADAPDKAIAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAE
VLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFEGAPLRGAIGLGVSLEGLPVG
FIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVI
AERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQK
YFSVENADGTLNARFVVVSNMDATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGK
VEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADASLVDRAALLAKADLTTGMVGE
FPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGF
WLIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADG
TSSDVIEHDLIAFIAERLKVQQREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLAS
EEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQDEERALSLALDTLLGEVSPSL
AAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIE
G