Protein Info for Rru_A2871 in Rhodospirillum rubrum S1H

Annotation: Peptidase M20D, amidohydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 TIGR01891: amidohydrolase" amino acids 17 to 375 (359 residues), 348.7 bits, see alignment E=2e-108 PF01546: Peptidase_M20" amino acids 76 to 385 (310 residues), 153.3 bits, see alignment E=8.5e-49 PF07687: M20_dimer" amino acids 189 to 277 (89 residues), 26.3 bits, see alignment E=6.2e-10

Best Hits

Swiss-Prot: 41% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to rru:Rru_A2871)

MetaCyc: 50% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RQC7 at UniProt or InterPro

Protein Sequence (391 amino acids)

>Rru_A2871 Peptidase M20D, amidohydrolase (NCBI) (Rhodospirillum rubrum S1H)
MTPAIPPAIAALTGDMKAWRHHLHAHPETAFEEHATADFIAGLLDDFGVEVHRGLAGTGV
VGVIAGKRTGNRAIGLRADIDALHVTEATGLPHASVHAGRMHACGHDGHTAMLLGAAKHL
AATRDFAGRLILIFQPAEENEGGGKVMVEEGLFDRFPVDAVYGMHNWPGLEEGHFALRTG
PIMAGYDVFEITLTGKGGHAAMPHLGTDQLVAAGHLMTALQSIVARSVNPTEAAVVSVTQ
MHGGDTWNVLPASVVLRGTVRTFTKAVQDLIETRITELSRSIAQGFGAEAAIHYERRYPA
TVNSPEEAAVAARVASAVVGADKVDTNCPQTMGAEDFAFMLGVKPGAYVQLGAGPGRGGC
MLHNPGYDFNDALLGVGASYWVGLVHDQLAG