Protein Info for Rru_A2710 in Rhodospirillum rubrum S1H

Annotation: Gram negative topoisomerase IV, subunit B (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 16 to 656 (641 residues), 768.8 bits, see alignment E=2.1e-235 PF02518: HATPase_c" amino acids 42 to 186 (145 residues), 58.6 bits, see alignment E=1.6e-19 PF00204: DNA_gyraseB" amino acids 243 to 412 (170 residues), 143.5 bits, see alignment E=9.7e-46 PF01751: Toprim" amino acids 440 to 539 (100 residues), 65.4 bits, see alignment E=8.4e-22 PF00986: DNA_gyraseB_C" amino acids 583 to 654 (72 residues), 72 bits, see alignment E=7.1e-24

Best Hits

Swiss-Prot: 61% identical to PARE_BARBK: DNA topoisomerase 4 subunit B (parE) from Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 100% identity to rru:Rru_A2710)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RQT8 at UniProt or InterPro

Protein Sequence (665 amino acids)

>Rru_A2710 Gram negative topoisomerase IV, subunit B (NCBI) (Rhodospirillum rubrum S1H)
MSDLFQDAAAAQPADAYTARSIEVLEGLEPVRRRPGMYIGGTDERALHHLVAEILDNAMD
EAVAGYAQVIDIDLAVDGSVCVRDNGRGIPIDPHPRFPDKSALEVILTTLHSGGKFGGDA
YKVSGGLHGVGISVVNALSDSLRVEVARDRTLWAQDFCRGAPLGPLQKIGPAPNRRGTQV
RFHPDAEIFGPRARFKPALLHRMARSKAYLFRGVRIRWSCAPELLPDDAGVPAEDVFHFP
NGLGDYLGHALANRPLVGGRTFAGTANLPDDMGTVEWALCWPEDDEPFFNSYVNTIPTPE
GGTHEAGLRNALTRGLRSFAEMSGNKKGAAALTAEDVVGGAAVMFSMFLRDPQFQGQTKE
KLASQEATRLVEQALRDPFDHWLTGAPDSANLLLTHALERADERLRRRQAKETSRKTATK
KLRLPGKLSDCTRSTRQGTELFLVEGDSAGGSAKQARDRQTQAVLPLRGKILNVASASLD
KLRGNQELSDLAEALGCGTRGAFDLEHLRYDKVVIMTDADVDGAHIASLLMTFFFREMPA
LVSAGHLYLAMPPLYRLTQGGTSVYARDDAHKDQLMKTVFKTKGKVEISRFKGLGEMPPA
QLRETTMAYATRQLLRVTVPGGKTEEDREDARETAKLVEELMGRKPELRFRFIQENARFA
ENLDI