Protein Info for Rru_A2556 in Rhodospirillum rubrum S1H

Annotation: Mandelate racemase/muconate lactonizing enzyme (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 transmembrane" amino acids 273 to 295 (23 residues), see Phobius details PF02746: MR_MLE_N" amino acids 6 to 119 (114 residues), 36.7 bits, see alignment E=4.4e-13 PF13378: MR_MLE_C" amino acids 141 to 294 (154 residues), 93 bits, see alignment E=2.3e-30

Best Hits

Swiss-Prot: 46% identical to AEEP_ECOLI: L-Ala-D/L-Glu epimerase (ycjG) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rru:Rru_A2556)

MetaCyc: 46% identical to L-Ala-D/L-Glu epimerase (Escherichia coli K-12 substr. MG1655)
RXN0-5228 [EC: 5.1.1.20]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RR89 at UniProt or InterPro

Protein Sequence (333 amino acids)

>Rru_A2556 Mandelate racemase/muconate lactonizing enzyme (NCBI) (Rhodospirillum rubrum S1H)
MTKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDE
TVEGVKADLAAMTEAVAEGLTPTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGL
AEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGADDPLGRVAAVRRGAPDSRLIV
DANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVP
RLARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAF
VDLDGPVLLARDREPGLRFTAGVIHPPGSELWG