Protein Info for Rru_A2441 in Rhodospirillum rubrum S1H

Annotation: Ribonucleoside-diphosphate reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1231 transmembrane" amino acids 512 to 530 (19 residues), see Phobius details amino acids 544 to 563 (20 residues), see Phobius details amino acids 570 to 587 (18 residues), see Phobius details PF08471: Ribonuc_red_2_N" amino acids 31 to 147 (117 residues), 138.9 bits, see alignment E=5.5e-45 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 132 to 753 (622 residues), 467.3 bits, see alignment E=4.1e-144 PF02867: Ribonuc_red_lgC" amino acids 198 to 751 (554 residues), 343.1 bits, see alignment E=2.9e-106 amino acids 802 to 915 (114 residues), 59.5 bits, see alignment E=2.6e-20

Best Hits

Swiss-Prot: 70% identical to NRDJ_BRADU: Vitamin B12-dependent ribonucleotide reductase (nrdJ) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 70% identity to bmf:BAB1_1063)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RRK4 at UniProt or InterPro

Protein Sequence (1231 amino acids)

>Rru_A2441 Ribonucleoside-diphosphate reductase (NCBI) (Rhodospirillum rubrum S1H)
MRIERHFTTDGQSPYAAIGFREAISEIRNPDGSVVFRADAVLVPDAWSQVATDVLAQKYF
RKAGIPARLRPVPEADVPEWLWRSEPDTEALESLPTEERLVGESDSRQVFDRLAGTWTYW
GWKGGYFDAESDAQAFFDEMRHMLATQVGAPNSPQWFNTGLHWAYGIDGPSQGHFYVDFK
TGEVKRSESAYEHPQPHACFIQSVNDDLVNDGGIMDLWVREARLFKYGSGTGTNFSSLRA
SGESLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVICDIDHPDIEEFITWKARE
EQKVAALVSGSKLCSQNLNAVMEATRTWDGKDGDERFDPTVNKTLKKAILGARKAMIPEN
YIKRVIQLSRQGFTGIDFETYDTDWDSEAYLTVAGQNSNNSVRVTDAFLQQVERDGAWTL
RHRMSEKTRTVKARTLWDEIAFAAWASADPGLQFDTTINDWHTCPESGRINASNPCSEYM
FLDDTACNLASLNLITFRGQGAEAGLFDAVGFAHACRLWTVVLEISVLMAQFPSREIAER
SYKFRTLGLGYANIGGLLMANGLPYDSDAGRALCGALSAIMTGVAYATSAEMAEQMGAFP
GFAINREAMLRVIRNHRRAAFGESKGYEALSIAPVPLDIRNCPQADLVAVAQRAWDKALE
IGTAHGFRNAQVSVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRMVPSA
LRTLGYGEAAIAAIINYAVGHATLKNAPGVNAQSLIAKGFTQDALARIEEGLASCFDIRF
AFNKWSLGEDFCRDVLKMSDAQLNDPGVDLLTTLGFSKADIEAANTHVCGAMTLEGAPGL
KDEHLAVFDCASPCGRLGKRFLSATSHIHMMAAAQPFISGAISKTINMPNEASVEDCKQA
YMTSWRLGLKANALYRDGSKLSQPLAAQVLEEDDDQETLEEAVAGKAVSVQAEVVAERIV
ERVIAKRSRLPDRRKGYTQKAIVGGHKVYLRTGEYEDGRLGEVFIDMHKEGAAFRSLMNN
FAIAISIGLQYGVPLEEYVEAFTFTRFEPQGMVQGNEAIKMATSILDYIFREVAISYLGR
NDLAHVEPEDLHPDTVGDGHEEGDLEGTWEEDHIATTVSAGYVRSNLYVLKHKHHHRENG
AVTAELAQSTGTHGATVGATSVSSSSMSSIASMSLGTGSGNGASDSKLSKIREARMKGYE
GDSCGECGNFTLVRNGTCMKCDTCGSTSGCS