Protein Info for Rru_A2320 in Rhodospirillum rubrum S1H

Annotation: Ankyrin (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 TIGR02317: methylisocitrate lyase" amino acids 15 to 296 (282 residues), 418.1 bits, see alignment E=8.2e-130 PF13714: PEP_mutase" amino acids 18 to 259 (242 residues), 160 bits, see alignment E=7.7e-51 PF00463: ICL" amino acids 88 to 179 (92 residues), 47.3 bits, see alignment E=1.2e-16

Best Hits

Swiss-Prot: 53% identical to MMGF_BACSU: 2-methylisocitrate lyase (mmgF) from Bacillus subtilis (strain 168)

KEGG orthology group: K01003, carboxyvinyl-carboxyphosphonate phosphorylmutase [EC: 2.7.8.23] (inferred from 100% identity to rru:Rru_A2320)

MetaCyc: 48% identical to 2-methylcitrate lyase (Cupriavidus necator)
Methylisocitrate lyase. [EC: 4.1.3.30]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.23 or 4.1.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RRX5 at UniProt or InterPro

Protein Sequence (306 amino acids)

>Rru_A2320 Ankyrin (NCBI) (Rhodospirillum rubrum S1H)
MPYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSAS
MGLPDLGILTIEDVAFFIRQIVRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHL
EDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVIIARTDAAASEGLEGAIARARL
YVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIW
PVSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIP
QGSPHR