Protein Info for Rru_A2319 in Rhodospirillum rubrum S1H

Annotation: 2-methylcitrate synthase/citrate synthase II (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 TIGR01800: 2-methylcitrate synthase/citrate synthase II" amino acids 13 to 380 (368 residues), 482.6 bits, see alignment E=3.2e-149 PF00285: Citrate_synt" amino acids 14 to 363 (350 residues), 405.8 bits, see alignment E=7.9e-126

Best Hits

Swiss-Prot: 77% identical to PRPC_CUPNE: 2-methylcitrate synthase (prpC) from Cupriavidus necator

KEGG orthology group: K01659, 2-methylcitrate synthase [EC: 2.3.3.5] (inferred from 100% identity to rru:Rru_A2319)

MetaCyc: 76% identical to 2-methylcitrate synthase (Cupriavidus necator)
2-methylcitrate synthase. [EC: 2.3.3.5]

Predicted SEED Role

"2-methylcitrate synthase (EC 2.3.3.5)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 2.3.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RRX6 at UniProt or InterPro

Protein Sequence (386 amino acids)

>Rru_A2319 2-methylcitrate synthase/citrate synthase II (NCBI) (Rhodospirillum rubrum S1H)
MTETTFKPKKSVALSGIAAGNTALCTVGRSGNDLHYRGYNILDIAETCEFEEIAHLLIHG
GLPTAAQYASYKAKLKVSRGLPRSVKEALETIPAAAHPMDVMRSGVSALGCALPEAYDHH
PVDARDIADRLIASLGSMLLYWYHYAHHGRRIEVETDEDSIGGHFLRLLHGKPPSDDFVQ
AMHTSLILYAEHEFNASTFTARVIAGTGSDMYSCISGAIGALRGPKHGGANEVAFGIQKR
YASLQHAEEDIRRRVEAKEVVIGFGHPVYTTADPRNAVIKRVAKGLADEVRSHLQYDVAE
RIEQVMWDTKAMFPNLDWYSAVSYHLMGVPTSMFTPLFVIARTCGWAAHVIEQRQDNKII
RPSANYTGPENLAFVPMSARSDHVSM