Protein Info for Rru_A2318 in Rhodospirillum rubrum S1H

Annotation: 2-methylcitrate dehydratase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 PF03972: MmgE_PrpD_N" amino acids 20 to 261 (242 residues), 225.9 bits, see alignment E=4.8e-71 PF19305: MmgE_PrpD_C" amino acids 286 to 470 (185 residues), 142.2 bits, see alignment E=1.5e-45

Best Hits

Swiss-Prot: 68% identical to PRPD1_CORGL: 2-methylcitrate dehydratase 1 (prpD1) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)

KEGG orthology group: K01720, 2-methylcitrate dehydratase [EC: 4.2.1.79] (inferred from 100% identity to rru:Rru_A2318)

Predicted SEED Role

"2-methylcitrate dehydratase (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RRX7 at UniProt or InterPro

Protein Sequence (499 amino acids)

>Rru_A2318 2-methylcitrate dehydratase (NCBI) (Rhodospirillum rubrum S1H)
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLAR
GPVRSARAQALGHRSERGAPIFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHP
GDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGICLHEHKIDHIAHLGPSAAGGI
GALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAK
RMGPKVGDLSQVERIVIQTSHHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQD
GGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHAKGADKAFGGKVIITLKGGRQ
IVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
LSFVVSPDTLGPATPPGIF