Protein Info for Rru_A2265 in Rhodospirillum rubrum S1H

Annotation: Electron transfer flavoprotein-ubiquinone oxidoreductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 PF01494: FAD_binding_3" amino acids 5 to 182 (178 residues), 35.2 bits, see alignment E=3e-12 PF12831: FAD_oxidored" amino acids 6 to 163 (158 residues), 31.7 bits, see alignment E=3.8e-11 PF01266: DAO" amino acids 6 to 53 (48 residues), 28.3 bits, see alignment 4.8e-10 PF00890: FAD_binding_2" amino acids 6 to 41 (36 residues), 25.9 bits, see alignment 2e-09 PF01134: GIDA" amino acids 6 to 164 (159 residues), 23.7 bits, see alignment E=8.6e-09 PF13450: NAD_binding_8" amino acids 9 to 40 (32 residues), 26 bits, see alignment (E = 3.3e-09)

Best Hits

Swiss-Prot: 65% identical to FIXC_AZOC5: Protein FixC (fixC) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00313, electron transfer flavoprotein-quinone oxidoreductase [EC: 1.5.5.-] (inferred from 100% identity to rru:Rru_A2265)

MetaCyc: 63% identical to quinone reductase (NADH,flavodoxin) complex electron transfer catalytic subunit (Azotobacter vinelandii)
1.19.1.M1 [EC: 1.19.1.M1]

Predicted SEED Role

"Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.5.5.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.19.1.M1 or 1.5.5.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RS30 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Rru_A2265 Electron transfer flavoprotein-ubiquinone oxidoreductase (NCBI) (Rhodospirillum rubrum S1H)
MAEHFDVIIVGAGMAGNAASYTLAKAGLNVLQIERGEYPGSKNVQGAILYANAVEQIIPG
FRDDAPLERHVIEQRMWMLDDRSYVGSHFRSENFNEEKPNRYTIIRAQFDKWFNRQARDA
GAMVICETTVLDLIRNDAGKVVGVRCDREGGEVYAEVVILCDGVNALVGQRSGIRSEISP
EHAALAVKEMHFLPREVINERFNVRDDEGVVIEALGTVTSGMLGTGFLYTNGESISLGIG
CIVSDFVETKIGPAKLLERFKAHPSIAPLIRGSEVKEYAAHLIPEGGYNAVPQLYGAGWL
ICGDAAHFLNAAHREGSNLALTSGRLAAETIIALKSEGKAMTEANLALYRQKLEESFVLK
DLKKYKDLPHTLWRNKQFVTTYPELMAKAAENFFRVDGVDKLTKEKDIVRSFRKARGVLG
LVGDAVKVARAWR