Protein Info for Rru_A2206 in Rhodospirillum rubrum S1H

Annotation: tartrate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05681: Fumerase
  • amino acids 60 to 334 (275 residues), 263 bits, E=2.9e-82
• PF05683: Fumerase_C
  • amino acids 342 to 547 (206 residues), 285.8 bits, E=1.5e-89
• TIGR00723: hydrolyase, tartrate beta subunit/fumarate domain protein, Fe-S type
  • amino acids 384 to 547 (164 residues), 187.3 bits, E=1e-59

Best Hits

Swiss-Prot: 65% identical to FUMA_SALTY: Fumarate hydratase class I, aerobic (fumA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01676, fumarate hydratase, class I [EC: 4.2.1.2] (inferred from 100% identity to rru:Rru_A2206)

MetaCyc: 65% identical to fumarase A (Escherichia coli K-12 substr. MG1655)
Oxaloacetate tautomerase. [EC: 5.3.2.2]; Fumarate hydratase. [EC: 5.3.2.2, 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class I (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• methylaspartate cycle (15/19 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• reductive TCA cycle I (9/11 steps found)
• incomplete reductive TCA cycle (6/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• pyruvate fermentation to propanoate I (5/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle II (8/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (9/14 steps found)
• L-glutamate degradation VIII (to propanoate) (5/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2 or 5.3.2.2

Sequence Analysis Tools

See Q2RS89 at UniProt or InterPro

Protein Sequence (548 amino acids)

>Rru_A2206 tartrate dehydrogenase (NCBI) (Rhodospirillum rubrum S1H)
MTDVSGDTRIMIDRAFADVFPLAEDKTLYRALEGTEGLVSVERFRGEEMLVVAPEALTRL
AQEAFRDIAHLLRPAHLSQLRAIMDDPEASANDRFVALELLKNANISAGMVLPMCQDTGT
AIIQGKKGQRVFSGDDDALALSEGVARTYQTLNLRYSQMAALSVFDEVNTGNNLPAQIDL
YATPGDAYKFLFMAKGGGSANKVFLYQQTKALLNPESLARFLDAQVRTLGTAACPPYHLS
IAIGGTSAEACLKAVKLASAKYLDGLPTTGGKDGHAFRDLALEAEVLAMTRAMGIGAQFG
GKYFCHDVRVVRLPRHGASCPVGIGVSCSADRQMLGKITAEGLFLEQLETNPAHYLPDVR
TEKLSAEVVKIDLSRPMAEIRATLSGYPIKTRVSLSGPMIVARDIAHAKLKERLDRGEGL
PEYFKNGAVYYAGPAKTPANYASGSFGPTTAGRMDAYVDLFQENGGSMVMIAKGNRSKAV
TEACKKHGGFYLGSIGGAAAQLALNSIRKVEVLEYPELGMEAIWRIEVEDFPAFIIVDDK
GNDFFQQL