Protein Info for Rru_A2129 in Rhodospirillum rubrum S1H

Annotation: Fumarate hydratase, class II (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 13 to 467 (455 residues), 711.4 bits, E=2.4e-218
• PF00206: Lyase_1
  • amino acids 20 to 349 (330 residues), 349.5 bits, E=1.9e-108
• PF10415: FumaraseC_C
  • amino acids 416 to 469 (54 residues), 68.6 bits, 4.9e-23

Best Hits

Swiss-Prot: 62% identical to FUMC_SYNY3: Fumarate hydratase class II (fumC) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 100% identity to rru:Rru_A2129)

MetaCyc: 61% identical to fumarase subunit (Ascaris suum)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• methylaspartate cycle (15/19 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• reductive TCA cycle I (9/11 steps found)
• incomplete reductive TCA cycle (6/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• pyruvate fermentation to propanoate I (5/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle II (8/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (9/14 steps found)
• L-glutamate degradation VIII (to propanoate) (5/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Sequence Analysis Tools

See Q2RSG6 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Rru_A2129 Fumarate hydratase, class II (NCBI) (Rhodospirillum rubrum S1H)
MPASPPPPAPATRRETDSMGAIDVPADRYWGAQTQRSLEHFSIGIERMPEALIRSFGTLK
EAAAFANRSLGQLDETRADLIVTAAREVAEGRLSEHFPLRVWQTGSGTQTNMNVNEVIAN
RAIELSGGVLGSKTPIHPNDHVNMAQSSNDTFPTAMHMAAVTALSCTLLPQVRGLRASLA
QKAKLFAPIVKIGRTHLQDAVPLTLGQEFSGYVAQLDQAIDRLEATLPGLRELALGGTAV
GTGLNTHPAFAVRAIARISEITGHPFRQADNLFAALAAHDAMVAASAGLRGLAVALTKIA
NDIRWLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCESLLMICLQVMAADGAVAMAGS
QGNFELNVHKPLIAYNVLQAITLLADGCKAFTHFLVEGLSADETRIAEMVDRSLMLVTAL
TPSIGYDRAAAIAKAAHANGTTLREETLASGLMDDAAFTAAVQPARMAHPHGTADDIDKD
GGGPRQG