Protein Info for Rru_A2051 in Rhodospirillum rubrum S1H

Annotation: Hydrophobe/amphiphile efflux-1 HAE1 (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1050 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 472 to 497 (26 residues), see Phobius details amino acids 539 to 557 (19 residues), see Phobius details amino acids 871 to 889 (19 residues), see Phobius details amino acids 896 to 916 (21 residues), see Phobius details amino acids 922 to 946 (25 residues), see Phobius details amino acids 971 to 991 (21 residues), see Phobius details amino acids 1003 to 1026 (24 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1036 (1036 residues), 1716.8 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1025 (1025 residues), 1318.5 bits, see alignment E=0 PF03176: MMPL" amino acids 300 to 496 (197 residues), 32.7 bits, see alignment E=6.3e-12

Best Hits

Swiss-Prot: 66% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 67% identity to abo:ABO_0964)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RSP4 at UniProt or InterPro

Protein Sequence (1050 amino acids)

>Rru_A2051 Hydrophobe/amphiphile efflux-1 HAE1 (NCBI) (Rhodospirillum rubrum S1H)
MASFFIDRPVFAWVIAIIIMLSGALSILFLPIEQYPTVAPPSVRINATYPGASAQTLENT
VTQVIEQQMNGLDNLRYISSTSDSSGSATITLTFNPEANPDIAQVQVQNKLQLATPLLPQ
EVQQQGIQVTKANNTFLMVVGFISKDGSLTESGIADFVSSNIKDPLSRTNGVGEVTVFGS
QHAMRIWLDPDKLISFGLTTTEVKSAITAQNAEVSAGQIGGAPSVPGQELNATIVAQTRL
ETVEEFGAILIRVNADGSQIRLRDIARIEIGAESYGTMARYNGQPASGIGVSLATGGNAL
DTADAVRATIERLRPIFPQSVDVIYPYDTTPFVKISIEEVVRTLGEAIILVFLVMYLFLQ
NIRATLIPTIAVPVVLLGTFGIMAAFGFTINTLTMFGLVLAIGLLVDDAIVVVENVERVM
HEDNLSPRDATRKSMRQITGALVGIALVLSAVFVPMAFFSGSVGAIYRQFSLTVASAMLL
SVLVAVVLTPALCATFLKPSHGDPYAKKGFFGWFNRGFDRTNSFYQSSVGHVIGRKWRYL
LIYGLILVGLGLLFVRLPSSFLPNEDQGILFAQASLPAGATQERTQEVLDQMSRHFRESE
ADFVEGVFTVNGFSFSGRGQNSGLAFVRMKNWDDRDPDKGSVFAVARRAMGALSRIKEAR
IFAFAPPAIIALGNASGFEFQLLDRAGQGHEKLMAARNQLLGMAAKNPALMAVRPDGLED
NPQLKIDIDQEKASALQLSLSDINSTLTTTWGSSYVNDFIENGRVKKVYLMADAPFRMQA
ADLDRWYVRNSQGEMVPFSAFTDVHWIYGSPRLERYNGQSSRKIAGQPAPGMSTGAAMAE
MEAMAAKLPPGFGYEWTGLSYEERLSGSQAPALYALSIIVVFLCLAALYESWAIPFAVIL
VVPLGILGAVIATSARGLSNDVFFQIGLLTTMGLAAKNAILIVEFAKELYEGGMDLLEAT
VHAARQRLRPIIMTSMAFSLGVLPLAIANGAGSGSQNAIGTGVLGGMISATVLAIFFVPL
FFILILRAFTKKTPSTPPAPPSPGAAADAP