Protein Info for Rru_A1989 in Rhodospirillum rubrum S1H

Annotation: Asparagine synthase, glutamine-hydrolyzing (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03104: asparagine synthase family amidotransferase
  • amino acids 1 to 589 (589 residues), 962 bits, E=9.2e-294
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 520 (519 residues), 417.7 bits, E=8.5e-129
• PF13522: GATase_6
  • amino acids 32 to 160 (129 residues), 143.9 bits, E=4.3e-46
• PF13537: GATase_7
  • amino acids 47 to 167 (121 residues), 148.8 bits, E=1.1e-47
• PF00733: Asn_synthase
  • amino acids 247 to 587 (341 residues), 301.6 bits, E=1.8e-93

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to rru:Rru_A1989)

MetaCyc: 67% identical to N-acetylglutaminylglutamine amide synthase (Sinorhizobium meliloti 1021)
RXN-13456

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• N-acetylglutaminylglutamine amide biosynthesis (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See Q2RSV6 at UniProt or InterPro

Protein Sequence (591 amino acids)

>Rru_A1989 Asparagine synthase, glutamine-hydrolyzing (NCBI) (Rhodospirillum rubrum S1H)
MCGVCGEIRFDGQFAGTTAVDRMTAKLAPRGPDDGGILVHGPVALGHRRLSIIDLSPKAA
QPMVDSDLGLSIVFNGCIYNYPALRSELEALGYRFFSGGDTEVILKAYHAWGTACVKRFN
GMFAFAIHERDSGKVVLARDRFGIKPLYLAEVDGGLLFASSLPALLATGRVDKAIDPLAL
RFYISFHAVVPAPRTILKGVRKLPPATLRVVSADGSSFDEVYWRLDFRALPGEDGRSAKD
WEDGLFEAVRAAVARRIIADVPVGVLLSGGLDSSLIVGLLAEAGQRDVETFSIGFEEANG
EKGDEFFYSDLIAERYATRHHKIFIPEADMIAALPGAIAAMSEPMVSYDNVAFYLLAREV
SKRVKVVLSGQGADEIFGGYHWYRPLVGSTDPVADYAAHFFDRTPERLARHLSPAWLGTT
DEARAFVAAHFAEDGAPDPVDKALRLDSTVMLVEDPVKRVDNMTMAFGLEARVPFLDHQL
AEFAGRIPSSLKLAQNGKGILKEVARRVVPAAVIDREKGYFPVPGLKYIEGSRLDYVRDG
LSSRAARQRGLFREDYLASLYENPRDAITPLRGSELWQVGLLELWLQEHGI