Protein Info for Rru_A1972 in Rhodospirillum rubrum S1H

Annotation: Phosphoenolpyruvate-protein phosphotransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 835 PF00359: PTS_EIIA_2" amino acids 11 to 145 (135 residues), 90.8 bits, see alignment E=2.1e-29 PF00381: PTS-HPr" amino acids 165 to 241 (77 residues), 74.5 bits, see alignment E=1.5e-24 TIGR01003: phosphocarrier, HPr family" amino acids 168 to 242 (75 residues), 53.2 bits, see alignment E=2.2e-18 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 276 to 831 (556 residues), 441.9 bits, see alignment E=3.1e-136 PF05524: PEP-utilisers_N" amino acids 278 to 396 (119 residues), 64.7 bits, see alignment E=2.3e-21 PF00391: PEP-utilizers" amino acids 425 to 497 (73 residues), 63.7 bits, see alignment E=2.3e-21 PF02896: PEP-utilizers_C" amino acids 523 to 807 (285 residues), 364.4 bits, see alignment E=1e-112

Best Hits

Swiss-Prot: 68% identical to PTFAX_XANCP: Multiphosphoryl transfer protein (fruB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to rru:Rru_A1972)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RSX3 at UniProt or InterPro

Protein Sequence (835 amino acids)

>Rru_A1972 Phosphoenolpyruvate-protein phosphotransferase (NCBI) (Rhodospirillum rubrum S1H)
MGSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFL
GHGVAIPHGMAEDRALVRANGIAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLT
RLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSDLAKRFEWVLDYPNGLHARPA
ARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKA
TMLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSV
PDHPVPLSEGGNILHEALSATRLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLT
CQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADLRDVGRRVLGQLDPGLGGASL
HDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPG
LLALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHR
VEIAANVNFADQAAFALSQGAEGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRP
LIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPDLLDPQLRALYRAAKSGKPLS
IMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGT
NDLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAA
LLTGLGVQELSMTPRDIPAVKERLRDCDATALRALAERALTCETATDVRALEADL