Protein Info for Rru_A1939 in Rhodospirillum rubrum S1H

Annotation: Pseudouridine synthase, Rsu (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 4 to 150 (147 residues), 64.4 bits, E=7.5e-22
• TIGR00093: pseudouridine synthase
  • amino acids 8 to 178 (171 residues), 166.9 bits, E=1.5e-53

Best Hits

Swiss-Prot: 61% identical to RLUE_ECO57: Ribosomal large subunit pseudouridine synthase E (rluE) from Escherichia coli O157:H7

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 100% identity to rru:Rru_A1939)

MetaCyc: 61% identical to 23S rRNA pseudouridine2457 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11834 [EC: 5.4.99.20]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.20

Sequence Analysis Tools

See Q2RT06 at UniProt or InterPro

Protein Sequence (199 amino acids)

>Rru_A1939 Pseudouridine synthase, Rsu (NCBI) (Rhodospirillum rubrum S1H)
MPRLILLNKPYDVLSQFTDRAEGRATLASLVPIPGVYVCGRLDRDSEGLLLLTDNGPLQH
HISHPRSKVPKTYRAQVEGVADKAALDRLRQGVTLRDGPARAVTVEAIDEPAGLWPRDPP
IRWRANIPTSWIELTIDEGRNRMVRRMTAAVGLPTLRLIRWSIGDWTLENLPPGSWREET
ASLPARRRPAAKPPRPPRR