Protein Info for Rru_A1915 in Rhodospirillum rubrum S1H

Annotation: DEAD/DEAH box helicase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 PF00270: DEAD" amino acids 26 to 195 (170 residues), 157.4 bits, see alignment E=4.3e-50 PF04851: ResIII" amino acids 46 to 191 (146 residues), 28.8 bits, see alignment E=1.7e-10 PF00271: Helicase_C" amino acids 233 to 342 (110 residues), 108.7 bits, see alignment E=2.8e-35

Best Hits

Swiss-Prot: 52% identical to RHLE_ECOLI: ATP-dependent RNA helicase RhlE (rhlE) from Escherichia coli (strain K12)

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 100% identity to rru:Rru_A1915)

MetaCyc: 52% identical to ATP-dependent RNA helicase RhlE (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase RhlE" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RT30 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Rru_A1915 DEAD/DEAH box helicase (NCBI) (Rhodospirillum rubrum S1H)
MTEFASLDLIEPLARAVTALGYTVATPIQAGAIPPQLLGRDVLGIAQTGTGKTASFVLPL
LQRLTKDPQPRVPGRPRALILTPTRELAQQITESIGDLGRTLSAIRCGVVVGGVDILRQT
RMVQRGLEILVATPGRLQDLMNRKAVDLRAIEVLVLDEADRMLDMGFAPAVKKIAAVLPR
KRQTVMFSATLPNEVTGLVASLMNDPVRVEVAPAASVANRIDQRVLFVEQTNKRKLLLDL
LGDARKVERAIVFTRTKHGANKLGIFLLEYGVKSDVIHGNKSQGARQRALNDFKSGKVRA
LVATDIAARGIDVDGITHVINFDLPNEPESYVHRIGRTARAGAAGIALSLCAPQEMPYLK
AIEKVIKSRIDVDSTHAFHTPSMARAHGSHGHR