Protein Info for Rru_A1889 in Rhodospirillum rubrum S1H
Annotation: Triosephosphate isomerase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to TPIS_BRASO: Triosephosphate isomerase (tpiA) from Bradyrhizobium sp. (strain ORS 278)
KEGG orthology group: K01803, triosephosphate isomerase (TIM) [EC: 5.3.1.1] (inferred from 100% identity to rru:Rru_A1889)Predicted SEED Role
"Triosephosphate isomerase (EC 5.3.1.1)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or MLST (EC 5.3.1.1)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
- Calvin-Benson-Bassham cycle (12/13 steps found)
- formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
- homolactic fermentation (11/12 steps found)
- glycolysis III (from glucose) (10/11 steps found)
- glycolysis IV (9/10 steps found)
- gluconeogenesis III (10/12 steps found)
- superpathway of anaerobic sucrose degradation (15/19 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
- glycolysis II (from fructose 6-phosphate) (9/11 steps found)
- ethene biosynthesis V (engineered) (19/25 steps found)
- oxygenic photosynthesis (13/17 steps found)
- gluconeogenesis I (10/13 steps found)
- glycolysis I (from glucose 6-phosphate) (10/13 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- glycerol degradation to butanol (12/16 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
- glycolysis VI (from fructose) (7/11 steps found)
- glycolysis V (Pyrococcus) (6/10 steps found)
- sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
- superpathway of hexitol degradation (bacteria) (11/18 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (10/17 steps found)
- superpathway of N-acetylneuraminate degradation (13/22 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
- Carbon fixation in photosynthetic organisms
- Fructose and mannose metabolism
- Glycolysis / Gluconeogenesis
- Inositol phosphate metabolism
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.1
Use Curated BLAST to search for 5.3.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RT56 at UniProt or InterPro
Protein Sequence (252 amino acids)
>Rru_A1889 Triosephosphate isomerase (NCBI) (Rhodospirillum rubrum S1H) MTVRRPLIAGNWKMNGLKAEGQGLARDLAQRLNKAGGVDFDLLLCPPFPLISTVVGAVAG SGIAVGGQDCHAKAKGAHTGDVSAPMLADLGCQFVIVGHSERRADHGETDALVKAKAEAA LAAGLIAVICVGETEAQRDAGETLAVVSGQLAGSLPTHADSGKVVIAYEPVWAIGTGRVP TTEDVAKVHGALRKDLVGALGADGNGVRLLYGGSVKPDNAAELMALENVDGALVGGASLK AEDFWAIAQSCA