Protein Info for Rru_A1881 in Rhodospirillum rubrum S1H
Annotation: Pyruvate dehydrogenase (lipoamide) (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to ODPA_RHIME: Pyruvate dehydrogenase E1 component subunit alpha (pdhA) from Rhizobium meliloti (strain 1021)
KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 100% identity to rru:Rru_A1881)MetaCyc: 39% identical to acetoin:DCPIP oxidoreductase alpha subunit (Syntrophotalea carbinolica DSM 2380)
RXN-9718 [EC: 2.3.1.190]
Predicted SEED Role
"Pyruvate dehydrogenase E1 component alpha subunit (EC 1.2.4.1)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
- TCA cycle I (prokaryotic) (9/10 steps found)
- TCA cycle III (animals) (9/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
- TCA cycle VIII (Chlamydia) (6/6 steps found)
- TCA cycle II (plants and fungi) (8/9 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
- pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- pyruvate fermentation to acetate II (3/3 steps found)
- pyruvate fermentation to acetate V (3/3 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- superpathway of glyoxylate bypass and TCA (9/12 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.1
Use Curated BLAST to search for 1.2.4.1 or 2.3.1.190
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RT64 at UniProt or InterPro
Protein Sequence (336 amino acids)
>Rru_A1881 Pyruvate dehydrogenase (lipoamide) (NCBI) (Rhodospirillum rubrum S1H) MAATRRKPRNGSSPATPTPDDIKGFYRDMLLIRRFEEKAGQLYGMGLIGGFCHLYIGQEA VVVGLQCQAHPGDSIITSYRDHGHMLAAGMDPKGVMAELTGRRGGYSKGKGGSMHMFSKE NGFYGGHGIVGAQVPLGTGLAFAHKYRGDGGVCFCYLGDGAANQGQVYESFNMAALWKLP VIYVIENNKYGMGTSVERASATKDLATRGAAYGIPGISVNGMDVLAVKAESEEAVDRVRA GEGPLILEMKTYRYRGHSMSDPAKYRTKEEVSKMRAESDPIDHLRQTIVSDAILDEEALK EIDKEIKSVVSQAAEFAQNSPEPDAAELYTDVLVEA